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AB50037

重组人IL-28A蛋白

Recombinant human IL-28A protein

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Recombinant human IL-28A protein is a Human Full Length protein, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, FuncS.

查看别名

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

1 Images
Western blot - Recombinant human IL-28A protein (AB50037)
  • WB

Lab

Western blot - Recombinant human IL-28A protein (AB50037)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209048 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-IL-28 + IL-29 antibody [EPR14608] (<a href='/products/primary-antibodies/hrp-il-28-il-29-antibody-epr14608-ab209048'>ab209048</a>) at 1/10000 dilution

All lanes:

Western blot - Recombinant human IL-28A protein (ab50037) at 0.1 µg

Predicted band size: 22 kDa

Observed band size: 20 kDa

true

Exposure time: 10s

关键信息

纯度

>98% SDS-PAGE

表达系统

Escherichia coli

标签

Tag free

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Biological activity of ab50037 is determined by its ability to activate STAT phosphorylation in an ISRE Luciferase Reporter Assay using human colon carcinoma COLO205 cells.

访问

Q8IZJ0

不含动物源

No

不含载体蛋白

No

种属

Human

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"PVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEAELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPRTRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8IZJ0","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
A few weeks
推荐的长期储存条件
-20°C
True
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特殊说明

形式

Lyophilized

附加说明

Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

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常规信息

功能

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

序列相似性

Belongs to the lambda interferon family.

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产品实验方案

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靶点信息

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2
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Primary Antibodies

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