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重组人IL-25蛋白(ab50145)

Submit a review Q&A (2)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

描述

  • 产品名称

    重组人IL-25蛋白
    参阅全部 IL-25 蛋白酶

  • 生物活性

    Determined by its ability to induce IL-8 in human PBMCs using a concentration range of 10-100 ng/ml.

  • 纯度

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • 表达系统

    Escherichia coli

  • 蛋白长度

    Protein fragment

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MYSHWPSCCP SKGQDTSEEL LRWSTVPVPP LEPARPNRHP ESCRASEDGP LNSRAISPWR YELDRDLNRL PQDLYHARCL CPHCVSLQTG SHMDPRGNSE LLYHNQTVFY RRPCHGEKGT HKGYCLERRL YRVSLACVCV RPRVMG

相关产品

技术指标

Our Abpromise guarantee covers the use of ab50145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

  • 形式

    Lyophilized

  • 补充说明

    This product was previously labelled as IL-17E

     

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Avoid freeze / thaw cycle. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

常规信息

  • 别名

    • IL 17E
    • IL 25
    • IL-17E
    • IL-25
    • IL17e
    • IL25
    • IL25_HUMAN
    • Interleukin 17E
    • Interleukin 25
    • Interleukin-17E
    • Interleukin-25
    • Interleukin17E
    • Interleukin25
    • OTTHUMP00000027946
    • OTTHUMP00000246238
    • UNQ3120/PRO10272
    see all

  • 功能

    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

  • 组织特异性

    Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

  • 序列相似性

    Belongs to the IL-17 family.

  • 细胞定位

    Secreted.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab50145？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50145 尚未被引用在任何文献中。

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

I wanted to get some information regarding the IL17E protein (ab50145). Which buffer was this protein dissolved in prior to lyophilisation?

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Answer

Thank you for contacting Abcam.


It appears that this information is lot specific. Do you already have a lot that you would like me to investigate? Otherwise I can check our current lot.


I look forward to your reply so that I may assist you further.

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Question

Dear technical support, I am wondering about which solution appropriate for reconstitution protein IL17e (ab 50145) lot 375844. Now I got your product in hand already but I can't reconstitute it. Because, in website and product insert sheet do not inform. Best regard,

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Answer

Thank you for your enquiry. Please centrifuge the vial prior to opening and reconstitute in water to a concentration of 0.1-1.0 mg/ml (do not vortex). This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC. I hope this helps and if I can assist further, please do not hesitate to contact me.

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