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生物活性等级

重组人IL-23蛋白(Active) (ab109153)

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Functional Studies - Recombinant human IL-23 protein (ab109153)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 0.060 Eu/µg
    • Active: Yes
    • Tags: DDDDK tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

    描述

    • 产品名称

      重组人IL-23蛋白(Active)
      参阅全部 IL-23 蛋白酶

    • 生物活性

      Induction of IL-17 secretion by mouse splenocytes.

    • 纯度

      > 90 % SDS-PAGE.

    • 内毒素水平

      < 0.060 Eu/µg

    • 表达系统

      HEK 293 cells

    • Accession

      Q9NPF7

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP

      • 预测分子量

        70 kDa including tags

      • 氨基酸

        23 to 328

      • 标签

        DDDDK tag N-Terminus

      • 额外的序列信息

        Fused at the C-terminus to a 18 aa linker peptide (GSTSGSGKPGSGEGSTKG) and to the IL23 p19 peptide (aa 20-189) and tagged at the N-terminus to a DDDDK-tag. IL-12p40 (human) Swissprot: P29460

    技术指标

    Our Abpromise guarantee covers the use of ab109153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Functional Studies

      SDS-PAGE

    • 形式

      Lyophilized

    • 补充说明

      Induction of IL17 secretion by Mouse splenocytes.

      PBS containing at least 0.1% BSA should be used for further dilutions.

      Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
      Add 1X PBS to the desired protein concentration.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.

      Constituent: 99% PBS

      0.2µm-filtered.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 复溶
      Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Add 1X PBS to the desired protein concentration.

    常规信息

    • 别名

      • IL 23
      • IL 23 A
      • IL 23 subunit alpha
      • IL 23A
      • IL 23p19
      • IL-23 subunit alpha
      • IL-23-A
      • IL-23p19
      • IL12B
      • IL23
      • Il23a
      • IL23A_HUMAN
      • IL23P19
      • interleukin 12B
      • Interleukin 23 alpha subunit p19
      • Interleukin 23 p19 subunit
      • interleukin 23 subunit alpha
      • interleukin 23 subunit p19
      • interleukin six, G CSF related factor
      • Interleukin-23 subunit alpha
      • Interleukin-23 subunit p19
      • JKA3 induced upon T cell activation
      • MGC79388
      • P19
      • SGRF
      see all

    • 功能

      Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

    • 组织特异性

      Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

    • 序列相似性

      Belongs to the IL-6 superfamily.

    • 发展阶段

      Expressed by newborns dendritic cells.

    • 细胞定位

      Secreted. Secreted upon association with IL12B.
    • Information by UniProt

    图片

    • Functional Studies - Recombinant human IL-23 protein (ab109153)
      Functional Studies - Recombinant human IL-23 protein (ab109153)

      IL17 secretion by Mouse splenocytes
      Mononuclear cells isolated from Mouse spleen were cultured with Mouse IL23 in presence or absence of various concentrations of ab109153 for 3 days. Levels of IL17 accumulated in culture supernatants were measured using ELISA.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab109153？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109153 尚未被引用在任何文献中。

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