重组人IL-1R-2蛋白(ab174069)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人IL-1R-2蛋白
参阅全部 IL-1R-2 蛋白酶 -
生物活性
Measured by its ability to inhibit IL1 beta dependent proliferation in D10.G4.1 mouse helper T cells.
Approximately 0.5-3 µg/ml of ab174069 will inhibit 50% of the biological response due to 50 pg/ml of recombinant Human IL1 beta. -
纯度
> 98 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
FTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSP RINLTWHKND SARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTR NASYCDKMSIELRVFENTDAFL PFISYPQILTLSTSGVLVCPDLSEFT RDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLL VHDVALEDAGYYRC VLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSR LT IPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENY IEVPLIFD PVTREDLHMDFKCVVHNTLSFQTLRTTVKE -
预测分子量
39 kDa including tags -
氨基酸
14 to 343 -
标签
His tag C-Terminus -
额外的序列信息
Extracellular domain. (Accession # NP_004624.1).
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
Previously labelled as IL1 Receptor II.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- IL1 Receptor II
- Antigen CDw121b
- CD121
see all -
功能
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1ra). -
序列相似性
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human IL-1R-2 protein (ab174069)Human IL-1 RII (His Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
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SDS-PAGE - Recombinant human IL-1R-2 protein (ab174069)SDS-PAGE analysis of reduced ab174069 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab174069 尚未被引用在任何文献中。
