重组人IgG4 Fc蛋白,Tag Free (Active) (ab181968)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE
描述
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产品名称
重组人IgG4 Fc蛋白,Tag Free (Active)
参阅全部 IgG4 蛋白酶 -
生物活性
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind ab181968 with an affinity constant of 0.715 μM as determined in SPR assay (Biacore).
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQ EDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKE YKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQ EGNVFSCSVMHEALHNHYTQKSLSLSLGK -
预测分子量
26 kDa -
氨基酸
99 to 327 -
额外的序列信息
Fc Region.
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描述
重组人IgG4蛋白(Active)
技术指标
Our Abpromise guarantee covers the use of ab181968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Surface Plasmon Resonance
SDS-PAGE
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形式
Lyophilized -
补充说明
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4
Constituents: 0.41% Tris, 0.51% Glycine, 6.8% Trehalose, 0.04% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 500 µg/ml.
常规信息
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别名
- Ig gamma 4 chain C region
- IGHG4
- immunoglobulin heavy chain constant region gamma 4
see all -
相关性
IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections. -
细胞定位
Secreted
图片
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SDS-PAGE of DDT-reduced (lane 1) and non-reduced (lane 2) ab181968 stained overnight with Coomassie Blue.
As a result of glycosylation, DTT-reduced (R) protein migrates as 30-32 kDa and non-reduced (NR) protein migrates as 28-30 and 55-60 kDa.
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Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind ab181968 with an affinity constant of 0.715 μM as determined in SPR assay (Biacore).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab181968 尚未被引用在任何文献中。