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生物活性等级

重组人IGFBP2蛋白(ab63223)

Submit a review Q&A (3)References (4)
Western blot - Recombinant human IGFBP2 protein (ab63223)

    Key features and details

    • Expression system: Insect cells
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: WB, Functional Studies, SDS-PAGE

    描述

    • 产品名称

      重组人IGFBP2蛋白

    • 生物活性

      Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.

    • 纯度

      > 98 % SDS-PAGE.
      Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg).

    • 内毒素水平

      < 1.000 Eu/µg

    • 表达系统

      Insect cells

    • Accession

      P18065

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ

      • 预测分子量

        32 kDa

      • 氨基酸

        37 to 325

      • 额外的序列信息

        The sequence is the full length mature form minus the signal peptide.

    技术指标

    Our Abpromise guarantee covers the use of ab63223 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Western blot

      Functional Studies

      SDS-PAGE

    • 形式

      Lyophilized
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 复溶
      For lot specific reconstitution information please contact our Scientific Support Team.

    常规信息

    • 别名

      • BP 2
      • BP2
      • IBP 2
      • IBP-2
      • IBP2
      • IBP2_HUMAN
      • IGF binding protein 2
      • IGF BP53
      • IGF-binding protein 2
      • IGFBP 2
      • IGFBP-2
      • IGFBP2
      • IGFBP53
      • Insulin like growth factor binding protein 2
      • Insulin like growth factor binding protein 2 36kDa
      • Insulin like growth factor-binding protein 2 precursor
      • Insulin-like growth factor-binding protein 2
      see all

    • 功能

      Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    • 序列相似性

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.

    • 结构域

      The C-terminus is required for IGF-binding and growth inhibition.

    • 翻译后修饰

      O-glycosylated.

    • 细胞定位

      Secreted.
    • Information by UniProt

    图片

    • Western blot - Recombinant human IGFBP2 protein (ab63223)
      Western blot - Recombinant human IGFBP2 protein (ab63223)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (4)

    发表研究结果有使用 ab63223？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab63223 被引用在 4 文献中.

    • Tan X  et al. A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. J Clin Invest 131:N/A (2021). PubMed: 32931483
    • Shimizu M  et al. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers (Basel) 13:N/A (2021). PubMed: 34572779
    • Li T  et al. IGFBP2 regulates PD-L1 expression by activating the EGFR-STAT3 signaling pathway in malignant melanoma. Cancer Lett 477:19-30 (2020). PubMed: 32120023
    • Chua CY  et al. IGFBP2 potentiates nuclear EGFR-STAT3 signaling. Oncogene 35:738-47 (2016). PubMed: 25893308

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: what is the recommended solution for reconstitution?

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    Answer

    I suggest to centrifuge the vial prior to opening. I recommend to reconstitute the Recombinant human IGFBP2 protein (ab63223) in water to a concentration of 0.1-1.0 mg/ml. Please do not vortex. This solution can be stored at 2°C-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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    Question

    How are the insect cells that are used to produce this product cultured - what media, is it serum free? Also, what is the possibility of contaminants in the product?

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    Answer

    A serum-free media is used for culturing. However, please note that the virus stock contains serum and the final concentration of the serum is 0.1% FBS in the production.

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    Question

    Customer kindly contact us to inquire whether this product was provided carrier protein free.

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    Answer

    This product is provided is free of any carrier proteins.

    Read More

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