Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
参阅全部 IGFBP1 蛋白酶
Biological Activity : The ED50, as determined by its ability to inhibit IGF-I induced proliferation of MCF-7, is < 0.5 µg/ml in the presence of 6 ng/ml of human IGF-I.
纯度> 98 % SDS-PAGE.
Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).
蛋白长度Full length protein
序列MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
Our Abpromise guarantee covers the use of ab50216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶Reconsitute to 1mg/ml with sterile water.
- Alpha pregnancy associated endometrial globulin
- Amniotic fluid binding protein
功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
序列相似性Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
翻译后修饰Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.