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AB116942

重组人 IDH3G 蛋白 (GST tag N-Terminus)

Recombinant Human IDH3G protein (GST tag N-Terminus)

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Recombinant Human IDH3G protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 393 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

Isocitric dehydrogenase subunit gamma, NAD(+)-specific ICDH subunit gamma, IDH3G

1 Images
SDS-PAGE - Recombinant Human IDH3G protein (GST tag N-Terminus) (AB116942)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human IDH3G protein (GST tag N-Terminus) (AB116942)

12.5% SDS-PAGE showing ab116942 at approximately 69.34kDa stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, SDS-PAGE, WB

applications

生物活性

No

访问

P51553

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }

序列信息

[{"sequence":"MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAASIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA","proteinLength":"Full Length","predictedMolecularWeight":"69.34 kDa","actualMolecularWeight":null,"aminoAcidEnd":393,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P51553","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The IDH3G protein also called isocitrate dehydrogenase 3 (NAD+) gamma is a subunit of the IDH3 enzyme complex. This protein has a molecular mass of approximately 39 kDa. It is highly expressed in tissues with a high energy demand like muscle cells and neurons. IDH3G plays an important role in the mitochondrial matrix where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate producing NADH and CO2 as byproducts.
Biological function summary

IDH3G is part of the IDH3 enzyme complex which contains multiple subunits and functions within mitochondria. This complex plays a critical role in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. This cycle is fundamental for energy production as it supplies reducing equivalents in the form of NADH which drive ATP synthesis through oxidative phosphorylation. The proper functioning of IDH3G contributes to efficient cellular respiration and energy homeostasis.

Pathways

The function of IDH3G connects to the TCA cycle and the energy metabolism pathway. Within the TCA cycle IDH3G's enzymatic activity ensures the continuation of the cycle and maintains the production of NADH. This is key for the generation of ATP in oxidative phosphorylation. The IDH3 complex works closely with the other components of the TCA cycle including succinate dehydrogenase and citrate synthase ensuring the continuity and regulation of metabolic flux in energy production pathways.

Defects or alterations in IDH3G are associated with metabolic disorders such as lactic acidosis and mitochondrial diseases. These conditions can result from impaired energy production and accumulation of metabolic intermediates. Furthermore IDH3G connections with related proteins could affect cell proliferation and survival in neurodegenerative diseases. Proper regulation and function of IDH3G may impact these diseases through its influence on energy metabolism pathways.

特殊说明

形式

Liquid

常规信息

功能

Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

序列相似性

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

亚细胞定位

Mitochondrion

产品实验方案

靶点信息

Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
See full target information IDH3G

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