重组人Hsp90 alpha蛋白(Active) (ab80369)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Hsp90 alpha蛋白(Active)
参阅全部 Hsp90 alpha 蛋白酶 -
生物活性
Kd app= 11.8 nM (lot specific).
Buffer contains 0.1M HEPES, pH 7.4, 0.25M MgCl2, 0.25M KCl, 0.1M Na2MoO4, and 0.05% Triton X. Incubated Hsp90alpha in a series dilution with 5 nM FITC-labeled geldanamycin for 150 minutes at room temperature.
Fluorescence polarization measured at ex 470/em528.
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纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLED PQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VDHHHHHH -
预测分子量
86 kDa including tags -
氨基酸
1 to 732 -
标签
His tag C-Terminus -
额外的序列信息
(NM_005348)
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab80369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.7% Imidazole
Constituents: 0.0463% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.632% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.638% Sodium chloride, 0.0165% Potassium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EL52
- epididymis luminal secretory protein 52
- Heat shock 86 kDa
see all -
功能
Molecular chaperone. Has ATPase activity. -
序列相似性
Belongs to the heat shock protein 90 family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
图片
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Functional Studies - Recombinant human Hsp90 alpha protein (ab80369)
Specific activity of ab80369.
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SDS-PAGE - Recombinant human Hsp90 alpha protein (Active) (ab80369)2 ug of ab80369 on 4-20% SDS-PAGE, stained with Coomassie.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab80369 被引用在 6 文献中.
- Pan X et al. Discovery of Quinacrine as a Potent Topo II and Hsp90 Dual-Target Inhibitor, Repurposing for Cancer Therapy. Molecules 27:N/A (2022). PubMed: 36080326
- Liu XY et al. Alkaloid Derivative (Z)-3β-Ethylamino-Pregn-17(20)-en Inhibits Triple-Negative Breast Cancer Metastasis and Angiogenesis by Targeting HSP90α. Molecules 27:N/A (2022). PubMed: 36296726
- Liu Z et al. Discovery of Small-Molecule Inhibitors of the HSP90-Calcineurin-NFAT Pathway against Glioblastoma. Cell Chem Biol 26:352-365.e7 (2019). PubMed: 30639261
- Li Z et al. Synthesis and biological evaluation of geldanamycin-ferulic acid conjugate as a potent Hsp90 inhibitor. RSC Adv 9:42509-42515 (2019). PubMed: 35542888
- Liang XH et al. Hsp90 protein interacts with phosphorothioate oligonucleotides containing hydrophobic 2'-modifications and enhances antisense activity. Nucleic Acids Res 44:3892-907 (2016). PubMed: 26945041
- Li Z et al. Discovery of novel 17-phenylethylaminegeldanamycin derivatives as potent Hsp90 inhibitors. Chem Biol Drug Des 85:181-8 (2015). PubMed: 24903735