重组人Hsp70蛋白(ab78433)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, ELISA, Functional Studies, Competitive Binding Assays, SDS-PAGE
描述
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产品名称
重组人Hsp70蛋白
参阅全部 Hsp70 蛋白酶 -
生物活性
ab78433 has ATPase activity at the time of manufacture of 3.0µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
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纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
AKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLI GDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKP KVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFN DSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLG GGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKK DISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRAR FEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQ DFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSL GLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTK DNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKI TITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKS AVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQ VCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD -
预测分子量
70 kDa -
氨基酸
1 to 641
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab78433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
Functional Studies
Competitive Binding Assays
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.242% Tris, 0.0154% DTT, 0.0475% Magnesium chloride, 0.375% Potassium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
相关性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
细胞定位
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
图片
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SDS-PAGE - Recombinant human Hsp70 protein (ab78433)SDS-PAGE of 70kDa Hsp70 protein.
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SDS-PAGE - Recombinant human Hsp70 protein (ab78433)Lane 1. Molecular weight ladder
Lane 2. Hsp70 protein
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab78433 被引用在 1 文献中.
- Goldberg-Murow M et al. Autoantibodies Against Ubiquitous and Confined Antigens in Patients With Ocular, Neuro-Ophthalmic and Congenital Cerebral Toxoplasmosis. Front Immunol 12:606963 (2021). PubMed: 34054794
