重组人Hsp60蛋白(ab113192)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: > 500.000 Eu/mg
- Active: Yes
- Suitable for: WB, EIA, PepArr, SDS-PAGE, Functional Studies, ELISA
描述
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产品名称
重组人Hsp60蛋白
参阅全部 Hsp60 蛋白酶 -
生物活性
ATPase activity assay (positive)but there is not quantification of activity
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纯度
> 90 % SDS-PAGE.
ab113192 is purified by multi-step chromatography. -
内毒素水平
> 500.000 Eu/mg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
60 kDa -
氨基酸
1 to 573
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab113192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Enzyme Immunoassay
Peptide Array
SDS-PAGE
Functional Studies
ELISA
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituent: 100% PBS
Dulbecco's PBS.This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
功能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
疾病相关
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
序列相似性
Belongs to the chaperonin (HSP60) family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
图片
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SDS-PAGE - Recombinant human Hsp60 protein (ab113192)SDS-PAGE showing ab113192 at approximately 60kDa (1µg).
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Western blot - Recombinant human Hsp60 protein (ab113192)All lanes : anti-Hsp60 mAb at 1 µg/ml
Lane 1 :Recombinant human Hsp60 protein (ab113192)
Lane 2 : GroEL protein
Lysates/proteins at 0.1 µg per lane.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab113192 尚未被引用在任何文献中。
