重组人HSF1 (phospho )蛋白(ab115508)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人HSF1 (phospho )蛋白
参阅全部 HSF1 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity =90% (~60% full-length with trace upstream kinase). -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
70 kDa including tags -
氨基酸
1 to 529 -
标签
His tag N-Terminus
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相关产品
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-HSF1 antibody (ab2923)
- Anti-HSF1 (phospho S303) antibody (ab47369)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-HSF1 antibody [10H8] (ab61382)
- Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
- Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)
技术指标
Our Abpromise guarantee covers the use of ab115508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: Tris buffered saline, 5% Glycerol (glycerin, glycerine)
常规信息
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别名
- Heat shock factor 1
- Heat shock factor protein 1
- Heat shock transcription factor 1
see all -
功能
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
序列相似性
Belongs to the HSF family. -
结构域
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻译后修饰
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human HSF1 (phospho ) protein (ab115508)SDS-PAGE analysis:
Lane 1: MWM,
Lane 2: 2µg HSF1,
Lane 3: 2µg ab115508 (HSF1 - phospho) -
Western blot - Recombinant Human HSF1 (phospho ) protein (ab115508)Western Blot analysis:
Lane 1: MW marker
Lane 2: HSF1
Lane 3: HSF1 (phospho) probed with HSF1 pAb -
Western blot - Recombinant Human HSF1 (phospho ) protein (ab115508)Western Blot analysis:
Lane 1: MW marker
Lane 2: HSF1
Lane 3: HSF1 (phospho) probed with HSF1 (pSer326)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115508 尚未被引用在任何文献中。