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AB268957

重组人hSET1/SET1蛋白(Active)

Recombinant human hSET1/SET1 protein (Active)

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Recombinant human hSET1/SET1 protein (Active) is a Human Fragment protein, in the 1418 to 1707 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA0339, KMT2F, SET1, SET1A, SETD1A, Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1/Ash2 histone methyltransferase complex subunit SET1, hSET1A

2 Images
Functional Studies - Recombinant human hSET1/SET1 protein (Active) (AB268957)
  • FuncS

Supplier Data

Functional Studies - Recombinant human hSET1/SET1 protein (Active) (AB268957)

The specific activity of ab268957 was 500 pmol/min/mg in a mehtyltransferase assay using histone H3 peptide (1-21) as substrate.

SDS-PAGE - Recombinant human hSET1/SET1 protein (Active) (AB268957)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human hSET1/SET1 protein (Active) (AB268957)

SDS-PAGE analysis of ab268957.

关键信息

纯度

>70% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

The specific activity of ab268957 was 500 pmol/min/mg in a mehtyltransferase assay using histone H3 peptide (1-21) as substrate.

访问

O15047

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"RSEFEQMTILYDIWNSGLDSEDMSYLRLTYERLLQQTSGADWLNDTHWVHHTITNLTTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGVDTQGTNRVLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1707,"aminoAcidStart":1418,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O15047","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

HSET1 also known as SET1 is a protein complex known for its role in histone methylation specifically on histone H3 at lysine 4 (H3K4). This methylation activity plays an important role in gene expression regulation. Predominantly expressed in the nucleus of eukaryotic cells hSET1 forms part of the COMPASS complex which weighs around 600 kDa. The enzyme encompasses various subunits each contributing to the overall stability and functionality of the complex.
Biological function summary

HSET1 influences chromatin structure modifications that regulate transcriptional activity. As a histone methyltransferase its activity results in epigenetic changes that turn genes on or off. hSET1 as part of the COMPASS complex recruits other factors to transcription initiation sites leading to active gene expression. This activity highlights its significant role in cell differentiation and development affecting how cells progress from one state to another.

Pathways

HSET1 plays a major role in the regulation of the Wnt signaling pathway and the DNA damage response pathway. Through the Wnt pathway hSET1 influences the dynamic process of cellular growth and differentiation interacting with proteins such as β-catenin. In the DNA damage response hSET1 modulates chromatin modifications facilitating the repair of damaged DNA coordinating with proteins like p53 to maintain genomic stability.

Aberrations in hSET1 functions associate with leukemias and certain neurodevelopmental disorders. In leukemia inappropriate activity or mutation in hSET1 leads to misregulated gene expression contributing to malignancy progression. Neurodevelopmental disorders have also shown correlations with abnormal hSET1 expression possibly affecting synaptic connectivity. Proteins such as EZH2 in the context of leukemia show interactions with SET1-related pathways highlighting its potential as a therapeutic target.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism (PubMed : 12670868, PubMed : 25561738). Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 29937342, PubMed : 31197650, PubMed : 32346159). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (By similarity) (PubMed : 31197650). Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation (PubMed : 29937342, PubMed : 32346159). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed : 12670868). Binds RNAs involved in RNA processing and the DNA damage response (PubMed : 38003223).

序列相似性

Belongs to the class V-like SAM-binding methyltransferase superfamily.

亚细胞定位

Nucleus speckle

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产品实验方案

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靶点信息

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism (PubMed : 12670868, PubMed : 25561738). Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 29937342, PubMed : 31197650, PubMed : 32346159). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (By similarity) (PubMed : 31197650). Required for H3K4me1 mark at stalled replication forks. Mediates FANCD2-dependent nucleosome remodeling and RAD51 nucleofilaments stabilization at reversed forks, protecting them from nucleolytic degradation (PubMed : 29937342, PubMed : 32346159). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed : 12670868). Binds RNAs involved in RNA processing and the DNA damage response (PubMed : 38003223).
See full target information SETD1A
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