重组人Hsc70蛋白(ab78431)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Affinity purified
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, Functional Studies, Competitive Binding Assays, SDS-PAGE
描述
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产品名称
重组人Hsc70蛋白
参阅全部 Hsc70 蛋白酶 -
生物活性
ab78431 has ATPase activity at the time of manufacture of 3.2 µM phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1 mM ATP using a Malachite Green assay.
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纯度
> 90 % Affinity purified.
ab78431 is affinity purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
73 kDa including tags -
氨基酸
1 to 646 -
标签
His tag N-Terminus -
额外的序列信息
His tag
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab78431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
Functional Studies
Competitive Binding Assays
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 8
Constituents: 1.753% Sodium chloride, 0.788% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 2410008N15Rik
- Constitutive heat shock protein 70
- Epididymis luminal protein 33
see all -
功能
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the heat shock protein 70 family. -
结构域
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
细胞定位
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
图片
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SDS-PAGE - Recombinant human Hsc70 protein (ab78431)SDS-PAGE of 73kDa Hsc70 protein.
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SDS-PAGE - Recombinant human Hsc70 protein (ab78431)Lane 1. Molecular weight ladder
Lane 2. Hsc70 protein
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (6)
ab78431 被引用在 6 文献中.
- Zhang KK et al. LAMP2A, and other chaperone-mediated autophagy related proteins, do not decline with age in genetically heterogeneous UM-HET3 mice. Aging (Albany NY) 15:4685-4698 (2023). PubMed: 37315291
- Endicott SJ et al. Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy. Autophagy 17:612-625 (2021). PubMed: 32013718
- Xu X et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell 12:769-787 (2021). PubMed: 34291435
- Fan Y et al. Acetylation-dependent regulation of TPD52 isoform 1 modulates chaperone-mediated autophagy in prostate cancer. Autophagy 17:4386-4400 (2021). PubMed: 34034634
- Endicott SJ et al. Inhibition of class I PI3K enhances chaperone-mediated autophagy. J Cell Biol 219:N/A (2020). PubMed: 33048163
- Labrador-Garrido A et al. Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the a-synuclein-elicited immune response. FASEB J 30:564-77 (2016). PubMed: 26443817
