重组人HPRT蛋白(Active) (ab222951)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE, Functional Studies
描述
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产品名称
重组人HPRT蛋白(Active)
参阅全部 HPRT 蛋白酶 -
生物活性
Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 µmole of guanosine 5’-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37°C.
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纯度
> 95 % SDS-PAGE.
ab222951 was purified using conventional chromatography techniques. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMATRSPGVVISDDEPGYDLDLFCIPNHYAE DLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLD YIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNV LIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVG FEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA -
预测分子量
27 kDa including tags -
氨基酸
1 to 218 -
标签
His tag N-Terminus -
额外的序列信息
NP_000185.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab222951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Mass Spectrometry
SDS-PAGE
Functional Studies
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 20% Glycerol, 0.32% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- HGPRT
- HGPRTase
- HPRT 1
see all -
功能
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. -
通路
Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1. -
疾病相关
Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT) [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. -
序列相似性
Belongs to the purine/pyrimidine phosphoribosyltransferase family. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant human HPRT protein (Active) (ab222951)
15% SDS-PAGE analysis of 3 µg ab222951.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab222951 尚未被引用在任何文献中。