重组人HIV-1 gp120 (CN54)蛋白(ab174070)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人HIV-1 gp120 (CN54)蛋白 -
生物活性
Immobilized Human CD4 at 2 μg/mL (100 μL/well) can bind HIV-1 GP120 (ab174070), His Tag with a linear range of 0.5-63 ng/mL
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纯度
> 95 % SDS-PAGE.
Purified by Immobilized metal affinity chromatography. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
TVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVL ENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLECRQVNT TNATSSVNVTNGEEIKNCSFNATTEIRDKKQKVYALFYRLDIVPLEEERK GNSSKYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNG TGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTII VHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAYCNIKKD DWIRTLQRVGKKLAEHFPRRIINFTSPAGGDLEITTHSFNCRGEFFYCNT SSLFNSTYNPNDTNSNSSSSNSSLDITIPCRIKQIINMWQEVGRAMYAPP IEGNITCKSNITGLLLVRDGGVESNETEIFRPGGGDMRNNWRSELYKYKV VEIKPLGIAPTAAKRRVVEREKR -
预测分子量
54 kDa including tags -
氨基酸
34 to 506 -
标签
His tag C-Terminus
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描述
重组人HIV-1 gp120 (CN54)蛋白
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- Envelope glycoprotein gp160
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相关性
The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface By similarity. The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves.
图片
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SDS-PAGE - Recombinant Human HIV-1 gp120 (CN54) protein (ab174070)
SDS-PAGE analysis of reduced ab174070 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 65-110 kDa due to glycosylation.
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Functional Studies - Recombinant human HIV-1 gp120 (CN54) protein (ab174070)Immobilized Human CD4, Fc Tag at 2 μg/mL (100 μL/well) can bind ab174070 with a linear range of 0.5-63 ng/mL (QC tested).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab174070 被引用在 1 文献中.
- Seiler BT et al. Broad-spectrum capture of clinical pathogens using engineered Fc-mannose-binding lectin enhanced by antibiotic treatment. F1000Res 8:108 (2019). PubMed: 31275563
