重组人HIPK1蛋白(ab60867)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人HIPK1蛋白 -
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
156 to 555
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Hipk 1
- Hipk1
- HIPK1_HUMAN
see all -
功能
May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation. -
组织特异性
Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. Phosphorylated and activated by JNK1. -
细胞定位
Nucleus. Cytoplasm. Predominantly nuclear. - Information by UniProt
图片
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Functional Studies - Recombinant human HIPK1 protein (ab60867)The specific activity of HIPK1 (ab60867) was determined to be 79 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human HIPK1 protein (ab60867)SDS PAGE analysis of ab60867 -
SDS-PAGE - Recombinant human HIPK1 protein (ab60867)SDS PAGE analysis of ab60867 -
Functional Studies - Recombinant human HIPK1 protein (ab60867)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human HIPK1 protein (ab60867)ab60867 on SDS-PAGE, MW ~71 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60867 尚未被引用在任何文献中。