JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259401

重组人HGF蛋白(Active)

Recombinant human HGF protein (Active)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant human HGF protein (Active) is a Human Full Length protein, in the 32 to 728 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

查看别名

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF

4 Images
Mass Spectrometry - Recombinant human HGF protein (Active) (AB259401)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human HGF protein (Active) (AB259401)

Mass determination by ESI TOF.

Predicted mass is 79720.38 Da (+/- 10 Da by ESI TOF). Observed mass is 79724.93.

Functional Studies - Recombinant human HGF protein (Active) (AB259401)
  • FuncS

Supplier Data

Functional Studies - Recombinant human HGF protein (Active) (AB259401)

Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.

ED50is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3369519-1​

SDS-PAGE - Recombinant human HGF protein (Active) (AB259401)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human HGF protein (Active) (AB259401)

SDS-PAGE analysis of ab259401.

Additional lower MW bands are result of protein degradation during the production process and do not affect protein function.

HPLC - Recombinant human HGF protein (Active) (AB259401)
  • HPLC

Supplier Data

HPLC - Recombinant human HGF protein (Active) (AB259401)

HPLC analysis of ab259401.

关键信息

纯度

>95% SDS-PAGE

=95%  by HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

Cell Culture, SDS-PAGE, HPLC, FuncS, Mass Spec

applications

生物活性

Yes

生物学活性

Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.

ED50is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.

访问

P14210

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

搭配使用产品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Proteins & Peptides

AB285462

Recombinant human FGF2 protein (Active)

Bioactive

Functional Studies - Recombinant human FGF2 protein (Active) (AB285462)

  • 0
  • 0
Proteins & Peptides

AB270062

Recombinant human IGF1 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human IGF1 protein (Active) (AB270062)

  • 5
  • 1
Proteins & Peptides

AB259398

Recombinant human EGF protein (Active)

Premium Bioactive

Functional Studies - Recombinant human EGF protein (Active) (AB259398)

  • 5
  • 2
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

序列信息

[{"linker":null,"sequence":"QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS","proteinLength":"Full Length","predictedMolecularWeight":"79.72 kDa","actualMolecularWeight":"79.73 kDa","aminoAcidEnd":728,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P14210","tags":[]}]
style
left-column

性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
style
left-column

常规信息

功能

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).

序列相似性

Belongs to the peptidase S1 family. Plasminogen subfamily.

翻译后修饰

The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form (PubMed:20977675). The single-chain precursor undergoes proteolytic processing by HGFAC resulting in an active two-chain form (PubMed:8226803).

style
left-column

产品实验方案

style
left-column

靶点信息

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).
See full target information HGF
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39000441

2024

Differential Immune Checkpoint Protein Expression in HNSCC: The Role of HGF/MET Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Verena Boschert,Johannes Boenke,Ann-Kathrin Böhm,Jonas Teusch,Valentin Steinacker,Anton Straub,Stefan Hartmann
View all publications
style
left-column
style
left-column
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com