重组人Heme-regulated抑制剂蛋白(ab131665)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人Heme-regulated抑制剂蛋白 -
生物活性
The specific activity of ab131665 was determined to be 12 nmol/min/mg.
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纯度
> 70 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
120 kDa including tags -
氨基酸
1 to 630
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab131665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204878 (RS Repeat Peptide peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as EIF2AK1.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- E2AK1_HUMAN
- EC 2.7.11.1
- Eif2ak1
see all -
功能
Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions. -
组织特异性
Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level). -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 HRM (heme regulatory motif) repeats.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues. Autophosphorylation at Thr-488 is required for kinase activation. The active autophosphorylated form apparently is largely refractory to cellular heme fluctuations. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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The specific activity of Heme-regulated inhibitor (ab131665) was determined to be 14 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab131665
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The specific activity of ab131665 was determined to be 12 nmol/min/mg.
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SDS Page analysis of ab131665
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131665 被引用在 1 文献中.
- Alvarez-Castelao B et al. The switch-like expression of heme-regulated kinase 1 mediates neuronal proteostasis following proteasome inhibition. Elife 9:N/A (2020). PubMed: 32329716