重组人HDAC8蛋白(ab42633)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: Inhibition Assay
描述
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产品名称
重组人HDAC8蛋白
参阅全部 HDAC8 蛋白酶 -
生物活性
10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC. -
纯度
> 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab42633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
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形式
Liquid -
补充说明
Expressed in a Baculovirus infected Sf9 cell expression system. MW= 46.4 kDa. 10 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 100 uM Biomol substrate (Catalog number KI-178), and 15 ng/ul HDAC8. Incubation condition: 60 min at 30ºC. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride
常规信息
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别名
- CDA07
- CDLS5
- HD 8
see all -
功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
组织特异性
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
细胞定位
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab42633 尚未被引用在任何文献中。