重组人HDAC6蛋白(Active) (ab42632)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 65% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay, Functional Studies, SDS-PAGE
描述
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产品名称
重组人HDAC6蛋白(Active)
参阅全部 HDAC6 蛋白酶 -
生物活性
Specific Activity: ≥350 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature. -
纯度
> 65 % SDS-PAGE.
Protein was purified by affinity chromatography and gel filtration. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELG LEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAV LDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVT HPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYI AWPLQGWQATFGGGDH PPKSDPMGHHHHHHGRRRASVAAGILVPRGSP GLDGIYARGIQMTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGA VPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLV LDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKE ELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASG SVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQ QKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKA SNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLV LVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLR ALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLV RSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNM MNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTC HSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRL VEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGH ALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASS QIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSA GFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISE SMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKV EDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGE ESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTS EEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGAT LAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQG TTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADS MLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTI QENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAY VHHQALLDVKNIAHQNKFGEDMPHPH" -
预测分子量
161 kDa including tags -
氨基酸
1 to 1215 -
标签
GST tag N-Terminus -
额外的序列信息
BC069243. NP_006035 161 kDa including tags
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技术指标
Our Abpromise guarantee covers the use of ab42632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.0165% Potassium chloride, 0.04% Tween, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CPBHM
- FLJ16239
- HD 6
see all -
功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
翻译后修饰
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
细胞定位
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
图片
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Functional Studies - Recombinant human HDAC6 protein (Active) (ab42632)Specific Activity: ≥350 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
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SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632)4-20% SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632).
Lane 1: 2µg HDAC6.
Lane 2: MW marker.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab42632 被引用在 3 文献中.
- Huang Y et al. Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections. J Med Chem 61:6056-6074 (2018). PubMed: 29940115
- Zhang Y et al. Discovery of a fluorescent probe with HDAC6 selective inhibition. Eur J Med Chem 141:596-602 (2017). PubMed: 29102179
- Duan W et al. Design, synthesis, and antitumor evaluation of novel histone deacetylase inhibitors equipped with a phenylsulfonylfuroxan module as a nitric oxide donor. J Med Chem 58:4325-38 (2015). PubMed: 25906087