重组人HAO1/GOX蛋白(ab113144)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人HAO1/GOX蛋白
参阅全部 HAO1/GOX 蛋白酶 -
纯度
> 95 % SDS-PAGE.
ab113144 was purified using conventional chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLPRLICINDYEQH AKSVLPKSIYDYYRSGAN DEETLADNIAAFSRWKLYPRMLRNVAETDL STSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMML SSW ATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQLR MKNFETSTLSFSPEENFGDDSGLAAY VAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILV SNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALAL GAKAVFVGRPIVWGLAFQGEKGVQDV LEILKEEFRLAMALSGCQNVKV IDKTLVRKNPLAVSKI -
预测分子量
45 kDa including tags -
氨基酸
1 to 370 -
标签
His tag N-Terminus , DDDDK tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab113144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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质谱法
MALDI-TOF -
形式
Liquid -
补充说明
Protein previously labeled as HAO1.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 2.92% Sodium chloride
常规信息
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别名
- (S) 2 hydroxy acid oxidase
- Glycolate oxidase
- GOX
see all -
功能
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. -
组织特异性
Liver. -
通路
Organic acid metabolism; glycolate degradation; 3-phospho-D-glycerate from glycolate: step 1/4. -
序列相似性
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain. -
细胞定位
Peroxisome. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human HAO1/GOX protein (ab113144)15% SDS-PAGE analysis of ab113144 (3µg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab113144 被引用在 1 文献中.
- Wang M et al. High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1. Sci Rep 6:34060 (2016). PubMed: 27670739