Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
参阅全部 Growth Hormone 蛋白酶
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.2ng/ml.
纯度> 95 % SDS-PAGE.
Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
内毒素水平< 1.000 Eu/µg
蛋白长度Full length protein
序列FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQT SLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANS LVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNS HNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
氨基酸27 to 217
额外的序列信息Full length mature chain without signal peptide. NP_000506.
Our Abpromise guarantee covers the use of ab51232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.0134% PBS, 1.6016% Mannitol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
功能Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
疾病相关Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
序列相似性Belongs to the somatotropin/prolactin family.
- Information by UniProt
15% SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab51232, 3 µg).
Reducing conditions. Visualized by coomassie blue stain.
All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution
Lane 1 :
Recombinant human Growth Hormone protein (Active) (ab51232) at 0.1 µg
Lane 2 :
Recombinant human Growth Hormone protein (Active) (ab51232) at 0.01 µg
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51232 被引用在 1 文献中.
- Landowski CP et al. Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei. PLoS One 10:e0134723 (2015). PubMed: 26309247