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AB280333

重组人Growth Hormone蛋白(Active)

Recombinant human Growth Hormone protein (Active)

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Recombinant human Growth Hormone protein (Active) is a Human Full Length protein, in the 27 to 217 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, sELISA, HPLC.

查看别名

Somatotropin, Growth hormone, Growth hormone 1, Pituitary growth hormone, GH, GH-N, GH1

5 Images
Functional Studies - Recombinant human Growth Hormone protein (Active) (AB280333)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Growth Hormone protein (Active) (AB280333)

Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line. ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.

Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (AB280333)
  • sELISA

Lab

Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (AB280333)

Sandwich ELISA - Recombinant human Growth Hormone protein standard curve.

Background subtracted standard curve using Human Growth Hormone Antibody Pair - BSA and Azide free (ab241146) and Recombinant human Growth Hormone protein (ab280333) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (AB280333)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (AB280333)

Mass determination by ESI-TOF.

Predicted MW is 22186.1 Da (+/- 10 Da by ESI-TOF). Observed mass is 22187.15

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB280333)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Growth Hormone protein (Active) (AB280333)

SDS-PAGE analysis of ab280333.

HPLC - Recombinant human Growth Hormone protein (Active) (AB280333)
  • HPLC

Supplier Data

HPLC - Recombinant human Growth Hormone protein (Active) (AB280333)

HPLC analysis of ab280333.

关键信息

纯度

>95% SDS-PAGE

=95% Purity by HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

sELISA, FuncS, SDS-PAGE, Mass Spec, HPLC

applications

生物活性

Yes

生物学活性

Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line.

ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.

访问

P01241

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

  • 0
  • 0
Proteins & Peptides

AB9794

Recombinant Human/Murine/Rat BDNF protein (Active)

Bioactive

Western blot - Recombinant Human/Murine/Rat BDNF protein (Active) (AB9794)

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein (DDDDK tag N-Terminus)

Functional Studies - Recombinant human XIAP protein (DDDDK tag N-Terminus) (AB95933)

  • 0
  • 0
Proteins & Peptides

AB270062

Recombinant human IGF1 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human IGF1 protein (Active) (AB270062)

  • 5
  • 1
Primary Antibodies

AB155276

Anti-Growth Hormone antibody [EPR11047(B)]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR11047(B)] (AB155276)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF","proteinLength":"Full Length","predictedMolecularWeight":"22.19 kDa","actualMolecularWeight":"22.19 kDa","aminoAcidEnd":217,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01241","tags":[]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Growth Hormone (GH) also known as somatotropin is a polypeptide hormone with a mass of approximately 22 kDa. The pituitary gland synthesizes and secretes GH. Once synthesized GH circulates in the bloodstream and affects various tissues throughout the body. GH's expression is tightly regulated because of its significant roles in growth processes. Researchers might use anti-growth hormone antibodies and various hGH testing kits including ELISA to study its levels and functions.
Biological function summary

GH triggers growth and cell reproduction by exerting effects on multiple tissues. It binds to the growth hormone receptor which initiates a cascade of reactions that ultimately stimulates growth in muscle bone and cartilage. GH also contributes to protein synthesis and helps regulate metabolism. GH acts mostly independently but can interact with cytokines and other hormones.

Pathways

GH plays important roles in the regulation of the JAK-STAT signaling pathway and the IGF-1 signaling pathway. The JAK-STAT pathway is important for transmitting the signal of GH binding to its receptor into cellular responses. GH stimulates IGF-1 production in the liver and IGF-1 then acts in an endocrine manner to mediate much of GH’s growth-promoting activity. These interactions highlight the interconnectedness of GH with other hormones in growth signaling networks.

Several health conditions can arise from abnormal levels of GH. Dwarfism occurs due to GH deficiency while acromegaly results from GH excess. Clinical analysis might use hGH kits to measure hormone levels and aid in diagnosis. GH's role in such conditions links it to other hormones like insulin and glucagon that help regulate glucose levels further affecting metabolic processes. Recombinant forms of GH are used in therapy for these disorders providing avenues for treatment and management.
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常规信息

功能

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

序列相似性

Belongs to the somatotropin/prolactin family.

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产品实验方案

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靶点信息

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
See full target information GH1
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