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AB316442

重组人Granzyme A蛋白Standard (His tag)

Recombinant Human Granzyme A Protein Standard (His tag)

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Recombinant Human Granzyme A Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

CTLA3, HFSP, GZMA, Granzyme A, CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF

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Sandwich ELISA - Recombinant Human Granzyme A Protein Standard (His tag) (AB316442)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Granzyme A Protein Standard (His tag) (AB316442)

Sandwich ELISA with the capture antibody ab244622 dilution at 2 μg/mL and detector antibody ab244875 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human Granzyme A Protein Standard (His tag) (AB316442)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Granzyme A Protein Standard (His tag) (AB316442)

SDS-PAGE analysis of ab316442.

关键信息

纯度

>80% SDS-PAGE

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE, sELISA

applications

生物活性

No

访问

P12544

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Proteins & Peptides

AB114201

Recombinant Human Perforin protein (GST tag N-Terminus)

Western blot - Recombinant Human Perforin protein (GST tag N-Terminus) (AB114201)

  • 0
  • 0
Proteins & Peptides

AB307479

Recombinant Human Factor D/CFD Protein (His tag) (Active)

Premium Bioactive

Functional Studies - Recombinant Human Factor D/CFD Protein (His tag) (Active) (AB307479)

  • 0
  • 0
Primary Antibodies

AB288728

Anti-Granzyme K antibody [EPR24601-56] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Granzyme K antibody [EPR24601-56] - BSA and Azide free (AB288728)

  • 0
  • 0
Assay Kits

AB253213

Mouse Granzyme A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Granzyme A ELISA Kit (AB253213)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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序列信息

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"27.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P12544","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification His Tag
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme A also known as CTLA3 or GZMA is a serine protease with a role in immune cell-mediated cytotoxicity. It has a molecular weight of approximately 27 kDa. This protein is primarily expressed in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Granzyme A gets released into the immunological synapse upon recognition of target cells by these immune cells facilitating the induction of target cell death.
Biological function summary

Functions of Granzyme A extend to inducing apoptosis in target cells alongside its role in promoting inflammation. It operates independent of caspases and directly cleaves substrates in the cell to induce cell death making it different from the related Granzyme B. As part of the granzyme-perforin complex it enters target cells via perforin-created pores mediates reactions leading to substrate degradation and promotes DNA damage.

Pathways

Granzyme A gets involved in the granule exocytosis pathway which constitutes an important mechanism for CTLs and NK cells to kill infected or cancerous cells. The protein is also linked to the inflammatory response pathways where it can interact with other serine proteases. Related proteins like perforin and Granzyme B also engage in these pathways and they collectively work to trigger apoptosis and modulate immune responses.

Impairments or dysregulation of Granzyme A function can contribute to autoimmune diseases and certain viral infections. Autoimmune diseases such as lupus may demonstrate altered levels or activity of Granzyme A associated with tissue damage and inflammation. During viral infections Granzyme A in conjunction with perforin and Granzyme B aids in eliminating infected cells but alterations in this balance could lead to ineffective immune responses or excessive tissue damage.
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常规信息

功能

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 12819770, PubMed : 32299851, PubMed : 3257574, PubMed : 3262682, PubMed : 3263427). It cleaves after Lys or Arg (PubMed : 12819770, PubMed : 32299851). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed : 32299851, PubMed : 34022140, PubMed : 36157507, PubMed : 36899106). Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed : 12524539). Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed : 11555662, PubMed : 12628186, PubMed : 16818237).

序列相似性

Belongs to the peptidase S1 family. Granzyme subfamily.

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产品实验方案

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靶点信息

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 12819770, PubMed : 32299851, PubMed : 3257574, PubMed : 3262682, PubMed : 3263427). It cleaves after Lys or Arg (PubMed : 12819770, PubMed : 32299851). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed : 32299851, PubMed : 34022140, PubMed : 36157507, PubMed : 36899106). Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed : 12524539). Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed : 11555662, PubMed : 12628186, PubMed : 16818237).
See full target information GZMA
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