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AB132889

重组人 GNAQ 蛋白 (GST tag N-Terminus)

Recombinant Human GNAQ protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human GNAQ protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 359 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

查看别名

GAQ, GNAQ, Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q

1 Images
SDS-PAGE - Recombinant Human GNAQ protein (GST tag N-Terminus) (AB132889)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GNAQ protein (GST tag N-Terminus) (AB132889)

12.5% SDS-PAGE analysis of ab132889 stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB, SDS-PAGE

applications

生物活性

No

访问

P50148

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV","proteinLength":"Full Length","predictedMolecularWeight":"65.23 kDa","actualMolecularWeight":null,"aminoAcidEnd":359,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P50148","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

GNAQ also known as Guanine nucleotide-binding protein G(q) subunit alpha plays a role in cell signaling as a modulator or transducer in various transmembrane signaling systems. With a molecular weight of approximately 42 kDa GNAQ belongs to the Gq family of G proteins. It is widely expressed in human tissues such as the brain heart and placenta. The protein's main mechanism involves binding to GTP and GDP leading it to switch between inactive GDP-bound and active GTP-bound states.
Biological function summary

GNAQ influences multiple cell processes mediating the activation of phospholipase C-beta a critical enzyme in hydrolyzing phosphatidylinositol 45-bisphosphate (PIP2) to inositol trisphosphate and diacylglycerol. This action leads to the release of calcium ions from intracellular stores. GNAQ acts as part of a heterotrimeric G protein complex which requires coordination with beta and gamma subunits to achieve full functional capability. Its activity is critical for processes like cell proliferation and differentiation.

Pathways

GNAQ notably participates in the phosphoinositide signaling pathway and calcium signaling pathway. In the phosphoinositide signaling pathway GNAQ facilitates the link between G protein-coupled receptors (GPCRs) and downstream effectors such as phospholipase C-beta. Its activity couples with proteins like GNA11 another member of the Gq family which often shares overlapping functions and roles within these pathways. The calcium signaling pathway involves GNAQ-induced release of calcium ions contributing to various cellular events.

GNAQ mutations link to uveal melanoma and Sturge-Weber syndrome. Studies show that these mutations activate the MAPK pathway independently promoting uncontrolled cell growth seen in uveal melanoma. GNA11 closely related to GNAQ also frequently undergoes similar mutations in uveal melanoma. In Sturge-Weber syndrome mutation of GNAQ leads to abnormal capillary growth and neurological issues evidencing its pathogenic role in vascular malformations.
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特殊说明

形式

Liquid

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常规信息

功能

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNA11, required for heart development (By similarity).

序列相似性

Belongs to the G-alpha family. G(q) subfamily.

翻译后修饰

Palmitoylated by ZDHHC3 and ZDHHC7 (PubMed:19001095). Palmitoylation occurs in the Golgi and participates in the localization of GNAQ to the plasma membrane (PubMed:19001095).. (Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.. Histaminylated at Gln-209 residues by TGM2.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed : 37991948). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 37991948). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 37991948). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 37991948). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 37991948). Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation : activates phospholipase C-beta : following GPCR activation, GNAQ activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) (PubMed : 37991948). Required for platelet activation (By similarity). Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity (By similarity). Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs) (PubMed : 27852822). Together with GNA11, required for heart development (By similarity).
See full target information GNAQ
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Regulatory, integrative and comparative physiology 318:R38-R48 PubMed31596114

2019

Evidence that hindbrain astrocytes in the rat detect low glucose with a glucose transporter 2-phospholipase C-calcium release mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Richard C Rogers,Susan J Burke,J Jason Collier,Sue Ritter,Gerlinda E Hermann
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搭配使用产品

Primary Antibodies

AB210004

Anti-GNAQ antibody [EPR20978]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-GNAQ antibody [EPR20978] (AB210004)

  • 0
  • 0
Proteins & Peptides

AB113585

Recombinant Human GNAI1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GNAI1 protein (His tag N-Terminus) (AB113585)

  • 0
  • 0
Primary Antibodies

AB204951

Anti-PKHD1 antibody [8G12A1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (AB204951)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

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