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AB271532

重组人 Glycogenin 1 + Glycogen synthase 2 蛋白 (His tag N-Terminus + Strep C-Terminus)

Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (His tag N-Terminus + Strep C-Terminus)

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Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (His tag N-Terminus + Strep C-Terminus) is a Human Full Length protein, in the 1 to 350 aa range, expressed in Baculovirus infected Sf9 cells, with >69%, suitable for SDS-PAGE.

查看别名

Glycogen synthase 2, GYS2

1 Images
SDS-PAGE - Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (His tag N-Terminus + Strep C-Terminus) (AB271532)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (His tag N-Terminus + Strep C-Terminus) (AB271532)

SDS-PAGE analysis of 3.6 μg ab271532.

关键信息

纯度

>69% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

Tag free

应用

SDS-PAGE

applications

生物活性

No

访问

P54840

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Preservative: 1.7% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MTDQAFVTLTTNDAYAKGALVLGSSLKQHRTTRRLVVLATPQVSDSMRKVLETVFDEVIMVDVLDSGDSAHLTLMKRPELGVTLTKLHCWSLTQYSKCVFMDADTLVLANIDDLFDREELSAAPDPGWPDCFNSGVFVYQPSVETYNQLLHLASEQGSFDGGDQGILNTFFSSWATTDIRKHLPFIYNLSSISIYSYLPAFKVFGASAKVVHFLGRVKPWNYTYDPKTKSVKSEAHDPNMTHPEFLILWWNIFTTNVLPLLQQFGLVKDTCSYVNVLSDLVYTLAFSCGFCRKEDVSGAISHLSLGEIPAMAQPFVSSEERKERWEQGQADYMGADSFDNIKRKLDTYLQ","proteinLength":"Full Length","predictedMolecularWeight":"42 kDa","actualMolecularWeight":"42 kDa","aminoAcidEnd":350,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P46976","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"MLRGRSLSVTSLGGLPQWEVEELPVEELLLFEVAWEVTNKVGGIYTVIQTKAKTTADEWGENYFLIGPYFEHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQVHFGRWLIEGSPYVVLFDIGYSAWNLDRWKGDLWEACSVGIPYHDREANDMLIFGSLTAWFLKEVTDHADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDITVMVFFIMPAKTNNFNVETLKGQAVRKQLWDVAHSVKEKFGKKLYDALLRGEIPDLNDILDRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNNRTDRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSPDDSCNQLTKFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPDKFHVELTSPPTTEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEVEDERYDEEEEAERDRLNIKSPFSLSHVPHGKKKLHGEYKN","proteinLength":"Full Length","predictedMolecularWeight":"82 kDa","actualMolecularWeight":"82 kDa","aminoAcidEnd":703,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P54840","tags":[{"tag":"Strep","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glycogenin 1 also known as glycogen protein or glycogenin protein plays an essential role in the biosynthesis of glycogen. Glycogenin 1 initiates the primer strand for glycogen synthesis by self-glucosylation which is the first step in building a glycogen molecule. Glycogenin 1 typically has a molecular mass of approximately 37 kDa. It expresses primarily in tissues with high energy demands such as liver and skeletal muscle. Glycogen synthase 2 on the other hand is an enzyme involved in elongating the glycogen chain once initiated by Glycogenin 1 working closely together in glycogen metabolism.
Biological function summary

Glycogenin 1 and glycogen synthase 2 play significant roles in glycogen metabolism. Glycogenin 1 starts glycogen synthesis by adding glucose units to itself forming the core structure of glycogen granules. Meanwhile glycogen synthase 2 extends the alpha-14-glucosidic links further enlarging the glycogen molecules. Both are integral to glycogenesis the process of converting glucose into glycogen for storage. These proteins often function in a complex with other enzymes and regulatory proteins ensuring that glycogen synthesis occurs efficiently.

Pathways

Both glycogenin 1 and glycogen synthase 2 are central in glycogen biosynthesis and storage pathways. Glycogen metabolism tightly regulates glucose homeostasis in response to hormonal signals and energy needs. Glycogen phosphorylase an enzyme that breaks down glycogen is influenced by these pathways. Glycogen synthase and glycogenin interact with regulatory kinases and phosphatases which modify their activity in response to signals therefore fine-tuning glycogen levels in cells.

Understanding glycogenin 1 and glycogen synthase 2 interactions has implications in diseases such as Type II diabetes and Glycogen Storage Disease Type 0. Their dysfunction leads to improper glycogen synthesis and disorder in energy balance. For example reduced glycogen synthesis in liver cells can cause fasting hypoglycemia a symptom associated with Glycogen Storage Disease Type 0. The metabolic implications of these proteins link them to pathways with insulin signaling and glucose metabolism impacting proteins like insulin receptor substrate 1 (IRS1) and AMP-activated protein kinase (AMPK) which play roles in maintaining glucose homeostasis.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

序列相似性

Belongs to the glycosyltransferase 3 family.

翻译后修饰

Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme (By similarity). Phosphorylation at Ser-8 is not required for interaction with GYG1 (By similarity). Interaction with GYG1 does not regulate the phosphorylation at Ser-8 and Ser-641 (By similarity).

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产品实验方案

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靶点信息

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
See full target information GYS2

其他靶点

GYG1
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搭配使用产品

Proteins & Peptides

AB158615

Recombinant Human Glycogen synthase 1/GYS1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Glycogen synthase 1/GYS1 protein (GST tag N-Terminus) (AB158615)

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Proteins & Peptides

AB271531

Recombinant Mouse Glycogenin 1 + GYS1 protein (Strep tag C-Terminus, His tag N-Terminus)

SDS-PAGE - Recombinant Mouse Glycogenin 1 + GYS1 protein (Strep tag C-Terminus, His tag N-Terminus) (AB271531)

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Primary Antibodies

AB224552

Anti-Glycogen synthase 2 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)

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Proteins & Peptides

AB162883

Recombinant Human ELOVL2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ELOVL2 protein (GST tag N-Terminus) (AB162883)

  • 0
  • 0

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