重组人Glucose 6 Phosphate Dehydrogenase蛋白(ab126671)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人Glucose 6 Phosphate Dehydrogenase蛋白
参阅全部 Glucose 6 Phosphate Dehydrogenase 蛋白酶 -
生物活性
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C
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纯度
> 95 % SDS-PAGE.
Affinity purified -
表达系统
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL -
预测分子量
61 kDa including tags -
氨基酸
1 to 515 -
标签
His tag N-Terminus
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相关产品
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Related Products
- Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)
- Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab87230)
- Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034)
- Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)
技术指标
Our Abpromise guarantee covers the use of ab126671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.002% PMSF, 0.03% DTT, 0.32% Tris HCl, 0.06% EDTA, 20% Glycerol, 1.17% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- G6PD
- G6PD_HUMAN
- G6PD1
see all -
功能
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
组织特异性
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
通路
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
疾病相关
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
序列相似性
Belongs to the glucose-6-phosphate dehydrogenase family. -
翻译后修饰
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
图片
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SDS-PAGE - Recombinant human Glucose 6 Phosphate Dehydrogenase protein (ab126671)15% SDS-PAGE analysis of ab126671 (3µg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab126671 尚未被引用在任何文献中。