重组人GLP-1R蛋白(ab130280)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人GLP-1R蛋白
参阅全部 GLP-1R 蛋白酶 -
纯度
> 90 % Densitometry.
ab130280 is purified using Ni-NTA chromatography and filtered (0.4 µm). -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MKHHHHHHAS RPQGATVSLW ETVQKWREYR RQCQRSLTED PPPATDLFCN RTFDEYACWP DGEPGSFVNV SCPWYLPWAS SVPQGHVYRF CTAEGLWLQK DNSSLPWRDL SECEESKRGE RSSPEEQLLF LY -
预测分子量
16 kDa including tags -
氨基酸
24 to 145 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab130280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -80°C.
Constituent: 0.41% Sodium acetate
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复溶Add 0.1M Acetate buffer pH=4.0 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/mL.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
常规信息
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别名
- GLP
- GLP 1 R
- GLP 1 receptor
see all -
功能
This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. -
细胞定位
Cell membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human GLP-1R protein (ab130280)14% SDS-PAGE separation of ab130280:
Lane 1: M.W. marker – 97, 66, 45, 31, 21, 14 kDa
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab130280 被引用在 1 文献中.
- Ortiz-Muñoz G et al. Cystic fibrosis transmembrane conductance regulator dysfunction in platelets drives lung hyperinflammation. J Clin Invest 130:2041-2053 (2020). PubMed: 31961827
