重组人GITR蛋白(Active) (Biotin) (ab271528)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人GITR蛋白(Active) (Biotin)
参阅全部 GITR 蛋白酶 -
生物活性
The GITR:GITRL binding activity was performed in an inhibitor screening assay kit. In a 96-well plate coated with GITRL, ab271528 is incubated, followed by streptavidin-HRP and addition of an HRP substrate. Chemiluminescence produce is measured using a chemiluminescence reader.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG NKTHNAVCVP GSPPAE -
预测分子量
43 kDa including tags -
分子量信息
This protein runs at a higher molecular weight by SDS-PAGE due to glycosylation. -
氨基酸
26 to 161 -
标签
Avi tag C-Terminus , Fc tag C-Terminus -
额外的序列信息
Human IgG1 Fc
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偶联物
Biotin
技术指标
Our Abpromise guarantee covers the use of ab271528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Enzymatically biotin-labeled using Avi-tag™ technology.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Activation inducible TNFR family receptor
- Activation-inducible TNFR family receptor
- AITR
see all -
功能
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. -
组织特异性
Expressed in lymph node, peripheral blood leukocytes and weakly in spleen. -
序列相似性
Contains 3 TNFR-Cys repeats. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human GITR protein (Active) (Biotin) (ab271528)
SDS-PAGE analysis of 3 µg ab271528.
This protein runs at a higher molecular weight due to glycosylation.
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Functional Studies - Recombinant human GITR protein (Active) (Biotin) (ab271528)GITRL: GITR-biotin binding.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271528 尚未被引用在任何文献中。