AB114149

重组人 GFAP 蛋白 (GST tag N-Terminus)

Name: 重组人GFAP蛋白 (ab114149)| Abcam中文官网
Brand: Abcam Limited
SKU: AB114149
Price: 6396 CNY
Availability: InStock

Recombinant Human GFAP protein (GST tag N-Terminus)

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(6 Publications)

Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.

- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control

查看别名

Glial fibrillary acidic protein, GFAP

2 Images

Unknown

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

All lanes:

Anti-GFAP antibody - Astrocyte Marker (<a href='/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (ab114149) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human GFAP protein (AB114149)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GFAP protein (AB114149)

12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

应用

SDS-PAGE, WB, ELISA

applications

生物活性

访问

P14136

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab114149 can be used as a WB positive control in conjunction with <a href='/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>." } } }

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产品详情

Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.

Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.

Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Check out our ELISA protocol for more information here.

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序列信息

[{"sequence":"MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM","proteinLength":"Full Length","predictedMolecularWeight":"76 kDa","actualMolecularWeight":null,"aminoAcidEnd":432,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P14136","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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特殊说明

形式

Liquid

附加说明

Glutathione Sepharose 4 Fast Flow

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常规信息

功能

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

序列相似性

Belongs to the intermediate filament family.

翻译后修饰

Phosphorylated by PKN1.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Medicine 102:e32885 PubMed36820591

2023

The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Haokun Jia,Yonghan Chen,Yi Wang,Linwei Jia,Yaohui Tian,Hao Jiang

Lupus science & medicine 9: PubMed35705307

2022

Role of autoantibodies and blood-brain barrier leakage in cognitive impairment in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

John G Hanly,Alexandra Legge,Lyna Kamintsky,Alon Friedman,Javeria A Hashmi,Steven D Beyea,John Fisk,Antonina Omisade,Cynthia Calkin,Tim Bardouille,Chris Bowen,Kara Matheson,Marvin J Fritzler

Journal of neural transmission (Vienna, Austria : 1996) 129:545-555 PubMed35364741

2022

Plasma autoantibodies to glial fibrillary acidic protein (GFAP) react with brain areas according to Braak staging of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Gschmack,Camelia-Maria Monoranu,Hecham Marouf,Sarah Meyer,Lena Lessel,Raja Idris,Daniela Berg,Walter Maetzler,Frank Steigerwald,Jens Volkmann,Manfred Gerlach,Peter Riederer,Eleni Koutsilieri,Carsten Scheller

Nanoscale advances 4:1770-1778 PubMed35434521

2022

Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Mattia Ghirardello,Radhe Shyam,Xia Liu,Teodoro Garcia-Millan,Imke Sittel,Javier Ramos-Soriano,Kathreena M Kurian,M Carmen Galan

Neuropsychiatric disease and treatment 16:2633-2647 PubMed33177827

2020

Transcription Factor E2F1 Aggravates Neurological Injury in Ischemic Stroke via microRNA-122-Targeted Sprouty2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Wu,Zhiqiang Gao,Jiang Zhang

Biosensors & bioelectronics 75:420-6 PubMed26356762

2015

Microwave-accelerated bioassay technique for rapid and quantitative detection of biological and environmental samples.

Applications

Unspecified application

Species

Unspecified reactive species

Muzaffer Mohammed,Maleeha F Syed,Kadir Aslan

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组人 GFAP 蛋白 (GST tag N-Terminus)

Recombinant Human GFAP protein (GST tag N-Terminus)

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

SDS-PAGE - Recombinant Human GFAP protein (AB114149)

关键信息

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液