Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
参阅全部 gamma Synuclein/SNCG 蛋白酶
纯度> 95 % SDS-PAGE.
The protein coding region of ß-Synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. ß-Synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
蛋白长度Full length protein
序列MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD
氨基酸1 to 127
Our Abpromise guarantee covers the use of ab48712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.316% Tris HCl, 0.58% Sodium chloride
- Breast cancer specific gene 1 protein
- Breast cancer specific protein 1
功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
序列相似性Belongs to the synuclein family.
翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
- Information by UniProt
15% SDS-PAGE gel loaded with recombinant gamma Synuclein/SNCG protein.
Anti-gamma Synuclein/SNCG antibody (ab46201) at 0.5 µg/ml +
Recombinant Human gamma Synuclein/SNCG protein (ab48712) at 0.05 µg
Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab48712 被引用在 3 文献中.
- Le T et al. Chimera RNA interference knockdown of ?-synuclein in human cortical astrocytes results in mitotic catastrophe. Neural Regen Res 15:1894-1902 (2020). WB ; Human . PubMed: 32246638
- Winham CL et al. ?-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release. Neuroscience N/A:N/A (2019). PubMed: 31078687
- Surgucheva I et al. ?-Synuclein: seeding of a-synuclein aggregation and transmission between cells. Biochemistry 51:4743-54 (2012). PubMed: 22620680