重组人gamma Synuclein/SNCG蛋白(ab48712)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: WB, SDS-PAGE
描述
-
产品名称
重组人gamma Synuclein/SNCG蛋白
参阅全部 gamma Synuclein/SNCG 蛋白酶 -
纯度
> 95 % SDS-PAGE.
The protein coding region of ß-Synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. ß-Synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD -
氨基酸
1 to 127
-
技术指标
Our Abpromise guarantee covers the use of ab48712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Western blot
SDS-PAGE
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.316% Tris HCl, 0.58% Sodium chloride
常规信息
-
别名
- BCSG1
- Breast cancer specific gene 1 protein
- Breast cancer specific protein 1
see all -
功能
Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. -
组织特异性
Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. -
序列相似性
Belongs to the synuclein family. -
翻译后修饰
Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. -
细胞定位
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. - Information by UniProt
图片
-
15% SDS-PAGE gel loaded with recombinant gamma Synuclein/SNCG protein.
-
Anti-gamma Synuclein/SNCG antibody (ab46201) at 0.5 µg/ml +
Recombinant Human gamma Synuclein/SNCG protein (ab48712) at 0.05 µg
Secondary
Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (3)
ab48712 被引用在 3 文献中.
- Le T et al. Chimera RNA interference knockdown of ?-synuclein in human cortical astrocytes results in mitotic catastrophe. Neural Regen Res 15:1894-1902 (2020). WB ; Human . PubMed: 32246638
- Winham CL et al. ?-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release. Neuroscience N/A:N/A (2019). PubMed: 31078687
- Surgucheva I et al. ?-Synuclein: seeding of a-synuclein aggregation and transmission between cells. Biochemistry 51:4743-54 (2012). PubMed: 22620680