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生物活性等级

重组人Galectin 3蛋白(Active) (ab50236)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: HPLC, Functional Studies, SDS-PAGE

描述

  • 产品名称

    重组人Galectin 3蛋白(Active)
    参阅全部 Galectin 3 蛋白酶

  • 生物活性

    Chemotactic activity, as determined by its ability to chemoattract human blood monocytes, was observed at a concentration of 2.5 µg/ml with a peak response obtained at 250 µg/ml.

  • 纯度

    >= 98 % SDS-PAGE.
    >=98% HPLC analyses. Endotoxin Level: < 0.1 ng/µg of protein (< 1 EU/µg).

  • 内毒素水平

    < 1.000 Eu/µg

  • 表达系统

    Escherichia coli

  • Accession

    P17931

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI

    • 预测分子量

      26 kDa

    • 氨基酸

      2 to 250

    • 额外的序列信息

      Full length mature protein, without the initiator methionine.

相关产品

技术指标

Our Abpromise guarantee covers the use of ab50236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    HPLC

    Functional Studies

    SDS-PAGE

  • 形式

    Lyophilized
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

常规信息

  • 别名

    • 35 kDa lectin
    • Carbohydrate binding protein 35
    • Carbohydrate-binding protein 35
    • CBP 35
    • CBP35
    • Gal-3
    • GAL3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin 3 internal gene,included
    • Galectin-3
    • Galectin3
    • GALIG
    • GBP
    • IgE binding protein
    • IgE-binding protein
    • L 31
    • L 34
    • L-31
    • L-34 galactoside-binding lectin
    • L31
    • Laminin-binding protein
    • Lectin L-29
    • Lectin, galactose binding, soluble 3
    • LEG3_HUMAN
    • LGALS2
    • LGALS3
    • MAC 2 antigen
    • Mac-2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    • MGC105387
    see all

  • 功能

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.

  • 组织特异性

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

  • 序列相似性

    Contains 1 galectin domain.

  • 细胞定位

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab50236？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50236 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Inquiry: ab 50236 i would like to know the buffer the galectin3 is lyophilised into. thanks

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Answer

Thank you for your inquiry.
I can confirm that ab50236 (Galectin 3 protein) was lyophilized from 0.5x PBS, pH 7.2.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
I hope this information is helpful and wish you good luck with your research.

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