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重组人Fyn蛋白(ab84696)

Submit a review Q&A (2)References (1)
Functional Studies - Recombinant human Fyn protein (ab84696)
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
  • Functional Studies - Recombinant human Fyn protein (ab84696)
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
  • Western blot - Recombinant human Fyn protein (ab84696)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, WB, SDS-PAGE

描述

  • 产品名称

    重组人Fyn蛋白
    参阅全部 Fyn 蛋白酶

  • 生物活性

    Specific activity: 166nmol/min/mg.

  • 纯度

    > 90 % Densitometry.
    Affinity purified.

  • 表达系统

    Baculovirus infected Sf9 cells

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGCV QCKDKEATKL TEERDGSLNQ SSGYRYGTDP TPQHYPSFGV TSIPNYNNFH AAGGQGLTVF GGVNSSSHTG TLRTRGGTGV TLFVALYDYE ARTEDDLSFH KGEKFQILNS SEGDWWEARS LTTGETGYIP SNYVAPVDSI QAEEWYFGKL GRKDAERQLL SFGNPRGTFL IRESETTKGA YSLSIRDWDD MKGDHVKHYK IRKLDNGGYY ITTRAQFETL QQLVQHYSER AAGLCCRLVV PCHKGMPRLT DLSVKTKDVW EIPRESLQLI KRLGNGQFGE VWMGTWNGNT KVAIKTLKPG TMSPESFLEE AQIMKKLKHD KLVQLYAVVS EEPIYIVTEY MNKGSLLDFL KDGEGRALKL PNLVDMAAQV AAGMAYIERM NYIHRDLRSA NILVGNGLIC KIADFGLARL IEDNEYTARQ GAKFPIKWTA PEAALYGRFT IKSDVWSFGI LLTELVTKGR VPYPGMNNRE VLEQVERGYR MPCPQDCPIS LHELMIHCWK KDPEERPTFE YLQSFLEDYF TATEPQYQPG ENL

    • 标签

      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab84696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    Western blot

    SDS-PAGE

  • 形式

    Liquid

  • 补充说明

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名

    • C syn protooncogene
    • Fyn
    • FYN oncogene related to SRC FGR YES
    • FYN_HUMAN
    • OKT3 induced calcium influx regulator
    • P59 FYN
    • p59-Fyn
    • Protein tyrosine kinase fyn
    • Proto oncogene tyrosine protein kinase fyn
    • Proto-oncogene c-Fyn
    • Proto-oncogene Syn
    • Protooncogene Syn
    • SLK
    • Src like kinase
    • Src yes related novel gene
    • Src-like kinase
    • Src/yes related novel
    • SYN
    • Tyrosine kinase p59fyn T
    • Tyrosine kinase p59fyn(T)
    • Tyrosine-protein kinase Fyn
    see all

  • 功能

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

  • 组织特异性

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 细胞定位

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

图片

  • Functional Studies - Recombinant human Fyn protein (ab84696)
    Functional Studies - Recombinant human Fyn protein (ab84696)
    The specific activity of Fyn (ab84696) was determined to be 95 nmol/min/mg as per activity assay protocol and was equivalent to 141 nmol/min/mg as per radiometric assay
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS PAGE analysis of ab84696
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS PAGE analysis of ab84696
  • Functional Studies - Recombinant human Fyn protein (ab84696)
    Functional Studies - Recombinant human Fyn protein (ab84696)
  • SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS-PAGE - Recombinant human Fyn protein (ab84696)
    SDS Page analysis of ab84696
  • Western blot - Recombinant human Fyn protein (ab84696)
    Western blot - Recombinant human Fyn protein (ab84696)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab84696？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab84696 被引用在 1 文献中.

  • Wu F  et al. Acetylation-dependent coupling between G6PD activity and apoptotic signaling. Nat Commun 14:6208 (2023). PubMed: 37798264

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Inquiry: I would like to buy an anti-Fyn antibody made in mouse that detects fyn form human and mouse tissues. This product 1881 says it is like this but the picture of the blot shows a band higher than 59 KDa and also says it was used an anti-goat secondary antibody. could you clarify about all this?

Read More
Answer

Thank you for your inquiry.
The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
Therefore the band at ca 85 kDa is what we would expect.
If you are using a sample with endogenous Fyn the expected size is ca 59 kDa.
I hope this information is helpful and wish you good luck with your research.

Read More

Question

I have several questions about Fyn protein (Active) (ab84696).

1. What's the activity of Fyn protein and what' the method for measurement of the protein?

2. what's the difference between the recombinant protein and natural protein? Is the combinant protein justhave an additional tag?

3. There are two phosphorylation sites on the natural protein. How about the combinant protein? The same or not? If not, could you please tell mewhat's the detail phosphorylation sites on combinant protein?

Read More
Answer

Thank you for contacting Abcam.
The activity of this protein is 166nmol/min/mg and was assessed using the gold standard radiometric approach where a radio-labeled p33 was used to quantify the phosphorylation of the kinase to its substrate.
The link below has a good description explaining what recombinant proteins are, that you may find helpful:
http://www.recombinant-antibody.com/protein/

If there is anything else I can help you with, please let me know.

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