重组人FGF2蛋白(Active) (ab9596)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
描述
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产品名称
重组人FGF2蛋白(Active)
参阅全部 FGF2 蛋白酶 -
生物活性
Determined by a cell proliferation assay using Balb/c 3T3 cells.
The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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纯度
>= 95 % SDS-PAGE.
>= 95% HPLC Sterile filtered -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
AAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS -
预测分子量
17 kDa -
氨基酸
135 to 288
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技术指标
Our Abpromise guarantee covers the use of ab9596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Cell Differentiation
SDS-PAGE
Cellular Activation
HPLC
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0605% Tris, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute in 5mM Tris, pH 7.6, to 0.1-1.0 mg/ml.
常规信息
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别名
- Basic fibroblast growth factor
- Basic fibroblast growth factor bFGF
- BFGF
see all -
功能
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
组织特异性
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
序列相似性
Belongs to the heparin-binding growth factors family. -
翻译后修饰
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
细胞定位
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
图片
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Sandwich ELISA - Recombinant human FGF2 protein (ab9596)Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.
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Cell differentiation - Recombinant human FGF2 protein (ab9596)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab9596 被引用在 4 文献中.
- Wang H et al. Posaconazole inhibits the stemness of cancer stem-like cells by inducing autophagy and suppressing the Wnt/β-catenin/survivin signaling pathway in glioblastoma. Front Pharmacol 13:905082 (2022). PubMed: 36034873
- Söderlund Z et al. Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration. Commun Biol 5:1349 (2022). PubMed: 36482075
- Song H et al. Effect of fibroblast growth factor 2 on degenerative endplate chondrocyte: From anabolism to catabolism. Exp Mol Pathol 118:104590 (2021). PubMed: 33285208
- Dias MH et al. Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Mol Oncol 13:290-306 (2019). PubMed: 30422399
