重组人FGF2蛋白(ab61845)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人FGF2蛋白
参阅全部 FGF2 蛋白酶 -
生物活性
Specific activity of 3MIU/mg
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纯度
> 95 % SDS-PAGE.
Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
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技术指标
Our Abpromise guarantee covers the use of ab61845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
Add 0.1% HSA or BSA for long term storage at -20°C. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.164% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
常规信息
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别名
- Basic fibroblast growth factor
- Basic fibroblast growth factor bFGF
- BFGF
see all -
功能
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
组织特异性
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
序列相似性
Belongs to the heparin-binding growth factors family. -
翻译后修饰
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
细胞定位
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
图片
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Functional Studies - Recombinant human FGF2 protein (ab61845)Functional analysis of ab61845
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Cellular activation - Recombinant human FGF2 protein (ab61845)Dose-dependent proliferation of mouse BALB/c 3T3 cells with ab61845. Assays were carried out in triplicate.
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Western blot - Recombinant human FGF2 protein (ab61845)ab61845 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human FGF basic has a predicted MW of 16.5 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab61845 尚未被引用在任何文献中。
