重组人Fas Ligand蛋白(Active) (ab109359)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Fas Ligand蛋白(Active)
参阅全部 Fas Ligand 蛋白酶 -
生物活性
Induces apoptosis of human Jurkat T cells at a concentration of <1ng/ml in the presence of 0.1 to 1μg/ml TNF Ligands Enhancer. In the absence of TNF Ligands Enhancer, ab109359 is working at 50-100 fold higher concentrations.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 0.050 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
QLFHLQKELAELRESTSQMHTASSLEKQIGHPSPPPEKKELRKVAHLTGK SNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSC NNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLT SADHLYVNVSELSLVNFEESQTFFGLYKL -
预测分子量
33 kDa including tags -
氨基酸
103 to 281 -
标签
DDDDK tag N-Terminus -
额外的序列信息
Extracellular domain.
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相关产品
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Related Products
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-Fas Ligand antibody (ab134401)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763)
技术指标
Our Abpromise guarantee covers the use of ab109359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
常规信息
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别名
- ALPS1B
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
see all -
功能
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
疾病相关
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
N-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
细胞定位
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab109359 被引用在 1 文献中.
- Thiyagarajan D et al. IL-1ß Promotes a New Function of DNase I as a Transcription Factor for the Fas Receptor Gene. Front Cell Dev Biol 6:7 (2018). PubMed: 29468159
