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AB180278

重组人EXOSC8蛋白

Recombinant Human EXOSC8 protein

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Recombinant Human EXOSC8 protein is a Human Full Length protein, in the 1 to 276 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

OIP2, RRP43, EXOSC8, Exosome complex component RRP43, Exosome component 8, Opa-interacting protein 2, Ribosomal RNA-processing protein 43, p9, OIP-2

1 Images
SDS-PAGE - Recombinant Human EXOSC8 protein (AB180278)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human EXOSC8 protein (AB180278)

15% SDS-PAGE analysis of ab180278 (3μg).

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec

applications

生物活性

No

访问

Q96B26

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAGFKTVEPLEYYRRFLKENCRPDGRELGEFRTTTVNIGSISTADGSALVKLGNTTVICGVKAEFAAPSTDAPDKGYVVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCISPGKLVWVLYCDLICLDYDGNILDACTFALLAALKNVQLPEVTINEETALAEVNLKKKSYLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTIVMDEEGKLCCLHKPGGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVIKSMKPK","proteinLength":"Full Length","predictedMolecularWeight":"32.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":276,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96B26","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EXOSC8 also known as exosome component 8 forms part of the core of the exosome complex. This protein has a molecular weight of approximately 27 kDa. It is expressed in various tissues with enriched expression in bone marrow. EXOSC8 functions as an important structural component of the exosome complex aiding in the degradation and processing of RNA molecules.
Biological function summary

EXOSC8 plays a central role in RNA metabolism as part of the exosome complex which is essential for RNA processing and turnover. It assists in the degradation of both precursor mRNA and aberrantly processed RNA. The exosome complex typically includes nine core components with EXOSC8 acting alongside these to help maintain RNA stability and facilitate gene expression regulation.

Pathways

EXOSC8 is involved in RNA processing pathways essential for cellular function and survival. These pathways such as the RNA degradation pathway and mRNA surveillance pathway ensure the quality and regulation of RNA species. EXOSC8 interacts closely with other exosome components like EXOSC3 and EXOSC9 enhancing RNA degradation and contributing to cellular homeostasis.

Mutations in EXOSC8 link to neurological conditions notably pontocerebellar hypoplasia type 1B. This rare genetic disorder affects brain development and function leading to severe developmental delays. EXOSC8 mutations can disrupt normal exosome function impacting proteins like EXOSC3 which may worsen disease severity. Another disorder associated with EXOSC8 is autosomal recessive cerebellar ataxia where defective RNA processing contributes to the disease pathology.
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特殊说明

形式

Liquid

附加说明

ab180278 is purified using conventional chromatography.

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常规信息

功能

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.

序列相似性

Belongs to the RNase PH family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
See full target information EXOSC8
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