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AB101664

重组人 ERP27 蛋白 (His tag N-Terminus)

Recombinant Human ERP27 protein (His tag N-Terminus)

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Recombinant Human ERP27 protein (His tag N-Terminus) is a Human Full Length protein, in the 26 to 273 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

C12orf46, UNQ781/PRO1575, ERP27, Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, Inactive protein disulfide-isomerase 27

1 Images
SDS-PAGE - Recombinant Human ERP27 protein (His tag N-Terminus) (AB101664)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ERP27 protein (His tag N-Terminus) (AB101664)

ab101664 at 3 ug on an SDS-PAGE gel (15%)

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q96DN0

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":273,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96DN0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERP27 also known as Endoplasmic Reticulum Protein 27 is a protein component with a molecular mass of approximately 27 kDa. It resides within the endoplasmic reticulum (ER) of the cell where it participates in protein folding and processing. Expressed in many tissues ERP27 is part of the protein disulfide isomerase (PDI) family. Although its structural features do not include the typical thioredoxin-like active sites ERP27 influences protein maturation by assisting in the correct assembly and folding of nascent proteins.
Biological function summary

The protein exhibits important roles in the cellular stress response and quality control mechanisms within the endoplasmic reticulum. It does not form a stable complex with other proteins but interacts transiently to exert its function. ERP27 collaborates closely with other ER-resident proteins like ERP44 and the PDI family to enhance the checking and refolding of misfolded proteins maintaining cellular homeostasis.

Pathways

ERP27 participates directly in the unfolded protein response (UPR) pathway a critical mechanism activated during ER stress. This pathway involves ERP27's transient interactions within a network of proteins including ER chaperones and other PDIs to alleviate stress and restore normal function. Furthermore ERP27 plays a role in calcium homeostasis through these pathways interacting with proteins such as calnexin and calreticulin.

ERP27's impairment or dysregulation associates with neurodegenerative diseases like Alzheimer's and Parkinson’s. The protein's involvement in the UPR links it to cellular processes that are disrupted in these conditions. Moreover it connects to proteins such as amyloid precursor protein (APP) in Alzheimer's influencing amyloid-beta peptide production. In Parkinson’s interactions with parkin and related proteins highlight ERP27's potential implications in disease pathogenesis and progression.
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特殊说明

形式

Liquid

附加说明

purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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常规信息

功能

Specifically binds unfolded proteins and may recruit protein disulfide isomerase PDIA3 to unfolded substrates (PubMed : 16940051, PubMed : 23192347). Binds protein substrates via a hydrophobic pocket in the C-terminal domain (PubMed : 16940051, PubMed : 23192347). May play a role in the unfolded stress response (PubMed : 23192347).

序列相似性

Belongs to the protein disulfide isomerase family.

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产品实验方案

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靶点信息

Specifically binds unfolded proteins and may recruit protein disulfide isomerase PDIA3 to unfolded substrates (PubMed : 16940051, PubMed : 23192347). Binds protein substrates via a hydrophobic pocket in the C-terminal domain (PubMed : 16940051, PubMed : 23192347). May play a role in the unfolded stress response (PubMed : 23192347).
See full target information ERP27
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Primary Antibodies

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