重组人ERK1蛋白(ab105904)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
描述
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产品名称
重组人ERK1蛋白
参阅全部 ERK1 蛋白酶 -
生物活性
Specific activity 840 nmol/min/mg.
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纯度
> 90 % Densitometry.
ab105904 was determined to be >90% pure densitometry. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
72 kDa including tags -
氨基酸
1 to 379 -
标签
GST tag N-Terminus
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab105904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- ERK 1
- ERK-1
- ERK1
see all -
功能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
图片
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Functional Studies - Recombinant human ERK1 protein (ab105904)The specific activity of ERK1 (ab105904) was determined to be 715 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human ERK1 protein (ab105904)SDS PAGE analysis of ab105904 -
SDS-PAGE - Recombinant human ERK1 protein (ab105904)SDS PAGE analysis of ab105904 -
SDS-PAGE - Recombinant human ERK1 protein (ab105904)ab105904 was determined to be >90% pure by densitometry. -
Functional Studies - Recombinant human ERK1 protein (ab105904)The specific activity of ab105904 was determined to be 840 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105904 尚未被引用在任何文献中。