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AB276332

重组人Ephrin B2蛋白(Fc Chimera His Tag)

Recombinant human Ephrin B2 protein (Fc Chimera His Tag)

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Recombinant human Ephrin B2 protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 229 aa range, expressed in HEK 293 cells, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

查看别名

EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L

2 Images
Functional Studies - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (AB276332)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (AB276332)

Measured by its binding ability in a functional ELISA .

Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml.

SDS-PAGE - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (AB276332)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Ephrin B2 protein (Fc Chimera His Tag) (AB276332)

SDS-PAGE analysis of ab276332

关键信息

纯度

>97% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

HEK 293 cells

标签

Fc tag C-Terminus His tag C-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Measured by its binding ability in a functional ELISA .

Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml.

访问

P52799

不含动物源

No

不含载体蛋白

Yes

种属

Human

存储溶液

pH: 7.4 Constituents: PBS

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Biochemicals

AB120129

Y-27632 dihydrochloride, Rho kinase inhibitor

Functional Studies - Y-27632 dihydrochloride, Rho kinase inhibitor (AB120129)

  • 5
  • 2
Primary Antibodies

AB119323

Anti-Ephrin B2 (phospho Y316) antibody

Western blot - Anti-Ephrin B2 (phospho Y316) antibody (AB119323)

  • 0
  • 0
Biochemicals

AB120848

ML7, myosin light chain kinase inhibitor

Chemical Structure - ML7, myosin light chain kinase inhibitor (AB120848)

  • 0
  • 0
Biochemicals

AB120425

(±)-Blebbistatin, inhibitor of myosin II

Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (AB120425)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MAVRRDSVWKYCWGVLMVLCRTAISKSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFA","proteinLength":"Fragment","predictedMolecularWeight":"50.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":229,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P52799","tags":[{"tag":"Fc","terminus":"C-Terminus"},{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin B2 also called EFNB2 or ephrin-B2 is a transmembrane protein that plays important roles in cell signaling. The protein weighs approximately 37 kDa and it is expressed in various tissues like endothelial cells and neuronal tissues. Ephrin B2 binds to Eph receptors which are a part of the largest family of receptor tyrosine kinases. This interaction facilitates bidirectional signaling necessary for mediating cell communication.
Biological function summary

Ephrin-B2 serves as critical mediator for vascular development and nervous system maturation. It is an important player in angiogenesis where it functions through interaction with EphB receptors to regulate blood vessel formation and remodeling. Ephrin-B2 part of the larger ephrin family often acts in concert with other ephrins and Eph receptors to form signaling complexes that coordinate cell positioning and tissue architecture.

Pathways

The action of ephrin-B2 integrates into the Eph/ephrin signaling pathway and the VEGF signaling pathway. These pathways are important for cellular guidance and migration particularly in developing tissues. Ephrin B2's interaction with proteins like VEGF and its receptors emphasizes its role in angiogenic processes. Furthermore Ephrin-B2 interacts with other Eph receptor proteins to modulate cellular responses essential for proper vascular and neural functions.

Ephrin-B2's dysregulation associates with cancer and cardiovascular conditions. High expression of ephrin-B2 has been observed in tumor angiogenesis linking it to cancer progression through interactions with VEGF. Abnormal ephrin-B2 signaling may also relate to atherosclerosis involving disrupted endothelial cell function. These associations reveal ephrin-B2's potential as a therapeutic target for conditions involving aberrant vascular and cellular dynamics.
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常规信息

功能

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.

序列相似性

Belongs to the ephrin family.

翻译后修饰

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

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产品实验方案

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靶点信息

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
See full target information EFNB2
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