重组人Eph receptor B2蛋白(ab60851)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Eph receptor B2蛋白
参阅全部 Eph receptor B2 蛋白酶 -
生物活性
Specific Activity: 39 nmol/min/mg.
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纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
66 kDa -
氨基酸
570 to 987 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 570-end of Human Eph receptor B2 (cytoplasmic domain).
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- cek5
- Developmentally regulated EPH related tyrosine kinase
- DRT
see all -
功能
Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor. -
组织特异性
Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain. -
疾病相关
Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant human Eph receptor B2 protein (ab60851)The specific activity of Eph receptor B2 (ab60851) was determined to be 39 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Eph receptor B2 protein (ab60851)SDS PAGE analysis of ab60851 -
SDS-PAGE - Recombinant human Eph receptor B2 protein (ab60851)SDS PAGE analysis of ab60851 -
Functional Studies - Recombinant human Eph receptor B2 protein (ab60851)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Eph receptor B2 protein (ab60851)ab60851 on SDS-PAGE, MW ~66 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60851 尚未被引用在任何文献中。