重组人Elongin-B + Elongin C + VHL蛋白(Tagged) (ab271497)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Elongin-B + Elongin C + VHL蛋白(Tagged) -
生物活性
Assay Conditions: Various amounts of ab271497 were incubated in 10-ul reaction with ARV-771 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
序列
DVFLMIRRHK TTIFTDAKES STVFELKRIV EGILKRPPDE QRLYKDDQLL DDGKTLGECG FTSQTARPQA PATVGLAFRA DDTFEALCIE PFSSPPELPD VMKPQDSGSS ANEQAVQ -
预测分子量
14 kDa including tags -
氨基酸
2 to 118 -
标签
DDDDK tag N-Terminus -
额外的序列信息
GenBank: NM_007108
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氨基酸序列 2
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种属
Human -
序列
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
预测分子量
13 kDa including tags -
氨基酸
2 to 112 -
标签
His tag N-Terminus -
额外的序列信息
GenBank: NM_005648
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氨基酸序列 3
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种属
Human -
序列
PRRAENWDEA EVGAEEAGVE EYGPEEDGGE ESGAEESGPE ESGPEELGAE EEMEAGRPRP VLRSVNSREP SQVIFCNRSP RVVLPVWLNF DGEPQPYPTL PPGTGRRIHS YRGHLWLFRD AGTHDGLLVN QTELFVPSLN VDGQPIFANI TLPVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD -
预测分子量
25 kDa including tags -
氨基酸
2 to 212 -
标签
DDDDK tag N-Terminus -
额外的序列信息
GenBank: NM_000551
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技术指标
Our Abpromise guarantee covers the use of ab271497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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细胞定位
Von Hippel Lindau/VHL: Cytoplasm. Membrane. Nucleus. Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated and Cytoplasm. Nucleus. Equally distributed between the nucleus and the cytoplasm but not membrane-associated. TCEB2/Elongin-B: Nucleus. Elongin-C: Nucleus.
图片
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Functional Studies - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)
ab271497-BRD3 interaction.
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SDS-PAGE - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)SDS-PAGE analysis of ab271497.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271497 尚未被引用在任何文献中。