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AB80323

重组人 EHMT2/G9A 蛋白 (GST tag N-Terminus)

Recombinant human EHMT2/G9A protein (GST tag N-Terminus)

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Recombinant human EHMT2/G9A protein (GST tag N-Terminus) is a Human Fragment protein, in the 785 to 1210 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.

查看别名

BAT8, C6orf30, G9A, KMT1C, NG36, EHMT2, Histone-lysine N-methyltransferase EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Lysine N-methyltransferase 1C, Protein G9a, H3-K9-HMTase 3

2 Images
Functional Studies - Recombinant human EHMT2/G9A protein (GST tag N-Terminus) (AB80323)
  • FuncS

Supplier Data

Functional Studies - Recombinant human EHMT2/G9A protein (GST tag N-Terminus) (AB80323)

Image showing specific activity of ab80323.

SDS-PAGE - Recombinant human EHMT2/G9A protein (GST tag N-Terminus) (AB80323)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human EHMT2/G9A protein (GST tag N-Terminus) (AB80323)

SDS-PAGE showing ab80323 at approximately 74.6kDa (5μg).

关键信息

纯度

>80% SDS-PAGE

Affinity purified.

表达系统

Escherichia coli

标签

GST tag N-Terminus

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Activity: 22 pmol/min/μg. Assay conditions: Enzyme was incubated with 0.5 μM of biotinylated H3 peptide with 40 μM AdoMet in HMT buffer 1A in a 10-μl reaction. 5 μl of Anti-Mouse AlphaLISA acceptor beads (1:250) and 5 μl of Primary Antibody 7 (1:50) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 μl) and mixture was further incubated for 15 min at RT before counts were measured.

访问

Q96KQ7

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB185050

Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EHMT2/G9A antibody [EPR18894] - ChIP Grade (AB185050)

  • 5
  • 4
Assay Kits

AB303753

Human EHMT2/G9A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EHMT2/G9A ELISA Kit (AB303753)

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Proteins & Peptides

AB101208

Recombinant Human RQCD1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human RQCD1 protein (His tag N-Terminus) (AB101208)

  • 0
  • 0
Primary Antibodies

AB194299

Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667] (AB194299)

  • 5
  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"74.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":1210,"aminoAcidStart":785,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q96KQ7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EHMT2 also known as G9a is a methyltransferase enzyme with a molecular mass of approximately 165 kDa. It modifies histones by adding methyl groups to lysine 9 on histone H3 which results in chromatin structure changes and influences gene expression. EHMT2/G9a expresses in various tissues including brain heart and liver showing its widespread role in different physiological processes. As a well-studied protein researchers often analyze EHMT2 activity using assays like ELISA and western blot.
Biological function summary

EHMT2/G9a regulates gene expression by altering chromatin accessibility. It is part of a chromatin remodeling complex interacting with proteins such as ANMTA02. Through its methyltransferase activity it maintains an epigenetic silence state important for embryonic development differentiation and cellular identity. EHMT2/G9a plays a significant role in the repression of transcriptional activity of tumor suppressor genes and developmental genes.

Pathways

G9a functions in epigenetic signaling pathways and influences processes like the cell cycle and DNA damage repair. It interacts with proteins in the Polycomb repressive complex such as EZH2 to regulate gene silencing. Additionally in the DNA damage response pathway G9a can associate with histone deacetylases to stabilize chromatin and prevent unwarranted gene expression.

Aberrant regulation of EHMT2/G9a associates with cancer and neurodevelopmental disorders. In various cancers such as leukemia G9a suppresses tumor suppressor genes aiding tumor progression. Connections between G9a and proteins like DNMT1 highlight its role in maintaining a hypermethylation state in cancerous cells. In neurodevelopmental disorders altered expression of G9a affects synaptic plasticity linking it with cognitive impairments and autism spectrum disorders.
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常规信息

功能

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones (PubMed : 11316813, PubMed : 20084102). Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication (PubMed : 22387026). Also weakly methylates 'Lys-27' of histone H3 (H3K27me) (PubMed : 11316813). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also able to mono- and dimethylate histone H1-4 at 'Lys-26' (H1.4K26me1 and H1.4K26me2, respectively) (PubMed : 19144645). In addition to the histone methyltransferase activity, also methylates non-histone proteins : mediates dimethylation of 'Lys-373' of p53/TP53 (PubMed : 20118233). Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself (PubMed : 18438403).

序列相似性

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.

翻译后修饰

Methylated at Lys-185; automethylated.

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产品实验方案

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靶点信息

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones (PubMed : 11316813, PubMed : 20084102). Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication (PubMed : 22387026). Also weakly methylates 'Lys-27' of histone H3 (H3K27me) (PubMed : 11316813). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also able to mono- and dimethylate histone H1-4 at 'Lys-26' (H1.4K26me1 and H1.4K26me2, respectively) (PubMed : 19144645). In addition to the histone methyltransferase activity, also methylates non-histone proteins : mediates dimethylation of 'Lys-373' of p53/TP53 (PubMed : 20118233). Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself (PubMed : 18438403).
See full target information EHMT2
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