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AB156723

重组人 EED 蛋白 (His tag N-Terminus)

Recombinant Human EED protein (His tag N-Terminus)

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Recombinant Human EED protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 441 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

查看别名

Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED

1 Images
SDS-PAGE - Recombinant Human EED protein (His tag N-Terminus) (AB156723)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human EED protein (His tag N-Terminus) (AB156723)

15% SDS-PAGE analysis of ab156723 (3μg).

关键信息

纯度

>85% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

O75530

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR","proteinLength":"Full Length","predictedMolecularWeight":"52.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O75530","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The EED protein also known as embryonic ectoderm development plays an important mechanical role within the cell. It is a component of the polycomb repressive complex 2 (PRC2) that mediates transcriptional silencing of target genes. The molecular weight of EED protein is approximately 50 kDa. EED is expressed in various tissues and is a critical mediator in maintaining transcriptional repression necessary for embryonic stem cell identity and differentiation.
Biological function summary

EED functions as a repressor of gene transcription through the polycomb group complex. It interacts with other components of PRC2 such as EZH2 SUZ12 to establish and maintain chromatin modifications. EED acts as a scaffold allowing communication between other proteins essential in the assembly and function of PRC2. These interactions regulate histone methylation contributing to gene silencing.

Pathways

EED plays a central role in the regulation of the Wnt and Hedgehog pathways. These pathways are critical for controlling cell fate decisions and developmental processes. In the Wnt pathway EED in conjunction with other PRC2 members modulates β-catenin-mediated transcription. In the Hedgehog pathway EED impacts the Gli family of transcription factors underlining its importance in development and cellular proliferation regulation.

EED has been linked to various cancers and developmental disorders. Aberrant EED function and localization relate to overactivation of the Wnt pathway an aspect seen in colorectal cancer. EED also interacts with EHZ2 contributing to tumorigenesis through unchecked cell division and proliferation. In the context of Weaver syndrome mutations within EED are involved consolidating its important association with developmental anomalies.
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特殊说明

形式

Liquid

附加说明

ab156723 is purified using conventional chromatography techniques

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常规信息

功能

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

序列相似性

Belongs to the WD repeat ESC family.

翻译后修饰

Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
See full target information EED
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搭配使用产品

Primary Antibodies

AB240650

Anti-EED antibody [EPR23043-5] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated|Trial Size

Immunoprecipitation - Anti-EED antibody [EPR23043-5] - ChIP Grade (AB240650)

  • 4
  • 5
Proteins & Peptides

AB84717

Recombinant Human Cdk6 protein

SDS-PAGE - Recombinant Human Cdk6 protein (AB84717)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB124821

Anti-Cdk6 antibody [EPR4515]

KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (AB124821)

  • 5
  • 2

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