JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153508

重组人E3 ubiquitin-protein ligase MUL1

Recombinant Human E3 ubiquitin-protein ligase MUL1

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human E3 ubiquitin-protein ligase MUL1 is a Human Full Length protein, in the 1 to 352 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

C1orf166, GIDE, MAPL, MULAN, RNF218, MUL1, Mitochondrial ubiquitin ligase activator of NFKB 1, E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, Protein Hades, Putative NF-kappa-B-activating protein 266, RING finger protein 218, RING-type E3 ubiquitin transferase NFKB 1

1 Images
SDS-PAGE - Recombinant Human E3 ubiquitin-protein ligase MUL1 (AB153508)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human E3 ubiquitin-protein ligase MUL1 (AB153508)

ab153508 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

Q969V5

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

序列信息

[{"sequence":"MESGGRPSLCQFILLGTTSVVTAALYSVYRQKARVSQELKGAKKVHLGEDLKSILSEAPGKCVPYAVIEGAVRSVKETLNSQFVENCKGVIQRLTLQEHKMVWNRTTHLWNDCSKIIHQRTNTVPFDLVPHEDGVDVAVRVLKPLDSVDLGLETVYEKFHPSIQSFTDVIGHYISGERPKGIQETEEMLKVGATLTGVGELVLDNNSVRLQPPKQGMQYYLSSQDFDSLLQRQESSVRLWKVLALVFGFATCATLFFILRKQYLQRQERLRLKQMQEEFQEHEAQLLSRAKPEDRESLKSACVVCLSSFKSCVFLECGHVCSCTECYRALPEPKKCPICRQAITRVIPLYNS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":352,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q969V5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

E3 ubiquitin-protein ligase MUL1 also known as MULAN or MAPL is an important enzyme in cellular processes. It possesses a mass of approximately 45 kDa and functions as an E3 ligase which facilitates the transfer of ubiquitin to specific substrate proteins. This tagging mechanism plays a critical role in directing proteins for degradation through the proteasome pathway. MUL1 is expressed in various tissues including the heart brain and skeletal muscle highlighting its broad biological relevance. As a mitochondrial membrane protein MUL1 helps maintain mitochondrial integrity by targeting specific proteins for ubiquitination.
Biological function summary

MUL1 regulates numerous cellular processes such as apoptosis mitophagy and the mitochondrial dynamics by modulating the stability of target proteins. MUL1 operates independently and does not require a specific E2 enzyme for its function. Additionally it participates in cellular homeostasis by mediating the degradation of proteins that impact mitochondrial fusion and fission events. Since MUL1 forms part of the regulatory mechanisms governing cellular adaptation and survival its levels and activity are finely controlled within the cell.

Pathways

MUL1 is actively involved in the mitophagy and DNA damage response pathways. Its role in the mitophagy pathway links MUL1 to PINK1 and PARKIN proteins that are critical for the removal of damaged mitochondria. In the context of the DNA damage response MUL1 functions to reprogram metabolic pathways yielding protection against cellular stress. This activity associates it with proteins like p53 a well-known tumor suppressor anchoring its presence in mechanisms of cellular defense.

MUL1 presents potential connections to neurodegenerative diseases and cancer. In Parkinson's disease dysfunction in MUL1 activity can disrupt mitochondrial quality control especially when interacting with PINK1 and PARKIN aggravating neuronal damage. Moreover alterations in MUL1 expression can influence cancer progression by impacting the p53 pathway therefore affecting cell cycle and apoptosis regulation. These associations reveal the importance of MUL1's activity for cellular health and its potential as a therapeutic target for disease intervention.
style
left-column

特殊说明

形式

Liquid

style
left-column

常规信息

功能

Exhibits weak E3 ubiquitin-protein ligase activity (PubMed : 18591963, PubMed : 19407830, PubMed : 22410793). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed : 18591963, PubMed : 19407830, PubMed : 22410793). Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteasomal degradation (PubMed : 22410793). Mediates polyubiquitination of cytoplasmic TP53 at 'Lys-24' which targets TP53 for proteasomal degradation, thus reducing TP53 levels in the cytoplasm and mitochondrion (PubMed : 21597459). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (PubMed : 19407830). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial localization (PubMed : 18207745, PubMed : 18213395, PubMed : 19407830). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed : 24898855). May also be involved in the sumoylation of the membrane fission protein DNM1L (PubMed : 18207745, PubMed : 19407830). Inhibits cell growth (PubMed : 18591963, PubMed : 22410793). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis (PubMed : 23399697). Involved in the modulation of innate immune defense against viruses by inhibiting RIGI-dependent antiviral response (PubMed : 23399697). Can mediate RIGI sumoylation and disrupt its polyubiquitination (PubMed : 23399697).

翻译后修饰

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

亚细胞定位

Mitochondrion outer membrane

style
left-column

产品实验方案

style
left-column

靶点信息

Exhibits weak E3 ubiquitin-protein ligase activity (PubMed : 18591963, PubMed : 19407830, PubMed : 22410793). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed : 18591963, PubMed : 19407830, PubMed : 22410793). Can ubiquitinate AKT1 preferentially at 'Lys-284' involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteasomal degradation (PubMed : 22410793). Mediates polyubiquitination of cytoplasmic TP53 at 'Lys-24' which targets TP53 for proteasomal degradation, thus reducing TP53 levels in the cytoplasm and mitochondrion (PubMed : 21597459). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (PubMed : 19407830). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial localization (PubMed : 18207745, PubMed : 18213395, PubMed : 19407830). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed : 24898855). May also be involved in the sumoylation of the membrane fission protein DNM1L (PubMed : 18207745, PubMed : 19407830). Inhibits cell growth (PubMed : 18591963, PubMed : 22410793). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis (PubMed : 23399697). Involved in the modulation of innate immune defense against viruses by inhibiting RIGI-dependent antiviral response (PubMed : 23399697). Can mediate RIGI sumoylation and disrupt its polyubiquitination (PubMed : 23399697).
See full target information MUL1
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB209263

Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241]

RabMAb|Recombinant

Immunoprecipitation - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (AB209263)

  • 5
  • 2
Proteins & Peptides

AB269109

Recombinant Human Ubiquitin protein

SDS-PAGE - Recombinant Human Ubiquitin protein (AB269109)

  • 0
  • 0
Proteins & Peptides

AB269103

Recombinant human UBE2I / UBC9 protein (Active)

Functional Studies - Recombinant human UBE2I / UBC9 protein (Active) (AB269103)

  • 0
  • 0
Proteins & Peptides

AB198417

Recombinant human UBE3A protein

Functional Studies - Recombinant human UBE3A protein (AB198417)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com