重组人DYRK2蛋白(ab133138)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
描述
-
产品名称
重组人DYRK2蛋白 -
生物活性
The specific activity of ab133138 was determined to be 1250 nmol/min/mg.
-
纯度
> 75 % Densitometry. -
表达系统
Escherichia coli -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
67 kDa -
氨基酸
1 to 601
-
相关产品
-
Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab133138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Western blot
SDS-PAGE
Functional Studies
-
形式
Liquid -
补充说明
ab204857 (DYRKtide peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- 1810038L18Rik
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 2
- Dual specificity tyrosine phosphorylation regulated kinase 2
see all -
功能
Role in the regulation of cellular growth and/or development. Regulates TP53 by phosphorylation on Ser-46 to induce apoptosis in response to DNA damage, functioning downstream of ATM. Inactivates GYS1 by phosphorylation at Ser-641, and potentially also a second phosphorylation site, thus regulating glycogen synthesis. Phosphorylates EIF2B5 at Ser-544, enabling its subsequent phosphorylation and inhibition by GSK3, and may play a more general role in the priming of GSK3 substrates. -
组织特异性
Testis, after the onset of spermatogenesis. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on serine/threonine residues. -
细胞定位
Cytoplasm. Nucleus. Translocates into the nucleus following DNA damage. - Information by UniProt
图片
-
The specific activity of DYRK2 (ab133138) was determined to be 1100 nmol/min/mg as per activity assay protocol
-
SDS PAGE analysis of ab133138
-
SDS PAGE analysis of ab133138
-
SDS-PAGE analysis of ab133138.
-
The specific activity of ab133138 was determined to be 1250 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab133138 尚未被引用在任何文献中。