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重组人DUSP23蛋白(ab117235)

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SDS-PAGE - Recombinant Human DUSP23 protein (ab117235)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    描述

    • 产品名称

      重组人DUSP23蛋白

    • 纯度

      > 90 % SDS-PAGE.
      ab117235 is purified using conventional chromatography techniques.

    • 表达系统

      Escherichia coli

    • Accession

      Q9BVJ7

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MGSSHHHHHHSSGLVPRGSHMGVQPPNFSWVLPGRLAGLALPRLPAHYQF LLDLGVRHLVSLTERGPPHSDSCPGLTLHRLRIPDFCPPAPDQIDRFVQI VDEANARGEAVGVHCALGFGRTGTMLACYLVKERGLAAGDAIAEIRRLRP GSIETYEQEKAVFQFYQRTK

      • 预测分子量

        19 kDa including tags

      • 氨基酸

        1 to 150

      • 标签

        His tag N-Terminus

    技术指标

    Our Abpromise guarantee covers the use of ab117235 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Mass Spectrometry

      SDS-PAGE

    • 质谱法

      MALDI-TOF

    • 形式

      Liquid

    • 补充说明

      EC=3.1.3.48.
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    常规信息

    • 别名

      • Dual specificity phosphatase 23
      • Dual specificity protein phosphatase 23
      • DUS23_HUMAN
      • Dusp23
      • DUSP25
      • EC 3.1.3.16
      • EC 3.1.3.48
      • FLJ20442
      • LDP 3
      • LDP-3
      • LDP3
      • Low molecular mass dual specificity phosphatase 3
      • MOSP
      • RP11-190A12.1
      • VH1 like member Z
      • VH1 like phosphatase Z
      • VH1-like phosphatase Z
      • VHZ
      see all

    • 功能

      Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).

    • 组织特异性

      Widely expressed. Highly expressed in spleen, prostate, colon, adrenal gland, mammary gland, thyroid and trachea. Expressed at lower level in uterus, small intestine, bladder, bone marrow, brain, spinal cord and stomach.

    • 序列相似性

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 tyrosine-protein phosphatase domain.

    • 细胞定位

      Cytoplasm > cytosol. Nucleus. Mainly cytosolic. Also nuclear.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human DUSP23 protein (ab117235)
      SDS-PAGE - Recombinant Human DUSP23 protein (ab117235)
      15% SDS-PAGE showing ab117235 at approximately 18.8kDa (3µg).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab117235？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab117235 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Is this protein active?

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    Answer

    Thank you for contacting us. The activity for ab 113160 is as follows:

    Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

    The activity of all the other proteins has not yet been tested. As a rule it should say "active" in the titale if it is active, however when new testing occurs it is not always immediately put into the title, so if there are ever any proteins you interested in we can always check them for you since once in a while you find one that has been tested but not updated. We currently have a project in the works that will update this active status more immediately.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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