重组人DR5蛋白(Fc Chimera Active) (ab174009)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
描述
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产品名称
重组人DR5蛋白(Fc Chimera Active)
参阅全部 DR5 蛋白酶 -
生物活性
Measured by its binding ability in a functional ELISA.
Immobilized ab174009 at 10 μg/ml (100 μl/well ) can bind biotinylated TNFSF10 with a linear range of 2.0 - 35 ng/ml. -
纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ITQQD LAPQQRAAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KE -
预测分子量
41 kDa including tags -
氨基酸
56 to 182 -
额外的序列信息
Fused with Fc fragment of human IgG1 at the C-terminus. NP_003833.
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技术指标
Our Abpromise guarantee covers the use of ab174009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
常规信息
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别名
- Fas like protein
- Apoptosis inducing protein TRICK2A/2B
- Apoptosis inducing receptor TRAIL R2
see all -
功能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
组织特异性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
疾病相关
Squamous cell carcinoma of the head and neck -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)Human DR5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)Immobilized recombinant Human TNFSF10 at 5 µg/mL (100 µL/well) can bind Human DR5, Fc Tag with a linear range of 0.4-13 ng/mL. -
SDS-PAGE - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)SDS-PAGE analysis of reduced ab174009 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 46 kDa due to glycosylation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab174009 尚未被引用在任何文献中。
