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AB241444

重组人DOCK8蛋白(His tag)

Recombinant Human DOCK8 protein (His tag)

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Recombinant Human DOCK8 protein (His tag) is a Human Fragment protein, in the 560 to 729 aa range, expressed in Yeast, with >85%, suitable for SDS-PAGE.

查看别名

Dedicator of cytokinesis protein 8, DOCK8

1 Images
SDS-PAGE - Recombinant Human DOCK8 protein (His tag) (AB241444)
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Supplier Data

SDS-PAGE - Recombinant Human DOCK8 protein (His tag) (AB241444)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab241444.

关键信息

纯度

>85% SDS-PAGE

表达系统

Yeast

标签

10x His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

Q8NF50

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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搭配使用产品

Primary Antibodies

AB175208

Anti-DOCK8 antibody [EPR12511]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DOCK8 antibody [EPR12511] (AB175208)

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Proteins & Peptides

AB95395

Recombinant Human ketohexokinase protein (Tag Free)

SDS-PAGE - Recombinant Human ketohexokinase protein (Tag Free) (AB95395)

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  • 0
Proteins & Peptides

AB116891

Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus) (AB116891)

  • 0
  • 0
Primary Antibodies

AB211777

Anti-NR2F2 antibody [EPR18443]

RabMAb|Recombinant|Advanced Validation|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [EPR18443] (AB211777)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"RNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPEFLQEVYTAVTYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMHSAEKVPLQNPPIKWAEGHKGVFNIEVQAV","proteinLength":"Fragment","predictedMolecularWeight":"21.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":729,"aminoAcidStart":560,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"Q8NF50","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DOCK8 also known as Dedicator of Cytokinesis 8 weighs approximately 210 kDa. It acts mechanically as a guanine nucleotide exchange factor (GEF) for small Rho GTPases which help regulate actin cytoskeleton dynamics. DOCK8 is expressed widely particularly in hematopoietic cells such as lymphocytes. It also associates with cell membranes where it facilitates signaling pathways involved in immune cell function.
Biological function summary

DOCK8 actively participates in immune system regulation by impacting the cytoskeletal rearrangements necessary for proper signaling and movement of immune cells. DOCK8 engages with proteins like DOCK2 and DOCK3 forming a complex that modulates these cellular mechanisms. Its function enhances the proper survival and immune response activities of leukocytes assisting in immune surveillance and pathogen clearance.

Pathways

Several immune-related signaling pathways involve DOCK8 especially the Rac-dependent pathways that affect actin polymerization. DOCK8 interacts closely with the CRK-like protein (CRKL) to mediate these processes. These pathways highlight the interaction between DOCK8 and other proteins critical to immune cell function positioning DOCK8 as an important regulator in immune system pathways.

Mutations in the DOCK8 gene correlate with DOCK8 deficiency a form of combined immunodeficiency characterized by eczema recurrent infections and high risk for malignancies. This disorder directly connects DOCK8 with suboptimal immune function. Furthermore DOCK8's interaction with the WASP protein plays a role in disrupting immune system health contributing to the pathophysiology of this immunodeficiency.
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常规信息

功能

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).

序列相似性

Belongs to the DOCK family.

翻译后修饰

In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.

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产品实验方案

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靶点信息

Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed : 22461490, PubMed : 28028151). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed : 28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed : 25762780).
See full target information DOCK8
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